微小RNA-7过表达对人肺癌细胞体内外转移的影响  

The effects of microRNA-7 on the metastasis of human lung cancer 95D cells in vitro and in vivo

作　　者：朱顺飞 廖珍媛[1] 胡燕[1] 陈超[1] 李永菊[1] 刘思静[2] 罗军敏[1] 熊思东[1] 徐林[1] 

机构地区：[1]遵义医学院免疫学教研室暨贵州省免疫学研究生教育创新基地,贵州遵义563099 [2]遵义医学院麻醉学系,2011级学生贵州遵义563099

出　　处：《遵义医学院学报》2013年第5期401-405,共5页Journal of Zunyi Medical University

基　　金：国家自然科学基金项目(NO:81260398);教育部新世纪优秀人才计划项目(NO:NCET-12-0661);贵州省国际合作项目(NO:10C315)

摘　　要：目的探讨过表达miR-7对人肺癌细胞体内外转移的影响。方法将真核表达载体pcDNA3.1(-)-pri-miR-7(命名为p-miR-7)体外瞬时转染人肺癌95D细胞后,划痕法检测细胞的体外迁移情况;侵袭实验检测细胞侵袭能力的改变;建立人肺癌裸鼠体内转移模型,HE染色观察过表达miR-7对肺部肿瘤转移的影响;免疫荧光技术检测肿瘤转移相关蛋白Sema4C蛋白的表达。结果过表达miR-7可显著抑制人肺癌细胞的体外迁移和侵袭能力(P<0.05);与对照组相比,过表达miR-7组未观察到明显的肺部肿瘤细胞转移灶(P<0.05);免疫荧光结果显示,过表达miR-7组中,肿瘤细胞内Sema4C蛋白表达量显著降低(P<0.05)。结论过表达miR-7可以显著抑制人肺癌细胞体外及体内的转移能力,提示miR-7有望成为肺癌后续基因治疗的新靶点。Objective To investigate the effects of miR -7 overexpression on the metastasis of human lung cancer cells in vivo and vitro. Methods The eukaryotic expression vector of pcDNA3.1 encoding miR-7（ termed as p- miR-7） was transiently transfected into human lung cancer 95D ceils in vitro. The migration of ceils was observed by scratch assay; the invasion of cells was observed by invasion as- say. Moreover, human lung cancer metastatic model in nude mice was established. The metastasis of lung cancer cells was observed by HE staining. Finally, the expression of Sema4c was also detected by immunohistochemistry assay. Results Overexpression of miR -7 can significantly inhibit the metastasis and invasion of lung cancer cells in vitro （P 〈 0. 05 ）. Moreover, compared to the control group, the metastasis of cancer cells in miR - 7 overexpression cells was not observed in the lung （P 〈 0. 05 ）. Fi- nally, the expression levels of Sema4C protein were also remarkably decreased （ P 〈 0. 05 ）. Conclusion Overexpression of miR -7 could significantly inhibit the metastasis of human lung cancer ceils in vivo and vitro, indicating a new target for subsequent lung cancer gene therapy.

关 键 词：肺癌 miRNA-7 转移 侵袭 Sema4C 生物治疗 

分 类 号：R737.33[医药卫生—肿瘤]