SFTPB、TACSTD1、PVA基因联合检测非小细胞肺癌淋巴结微转移的研究  

Study of Combined Detection of SFTPB、TACSTD1 and PVA Gene in The Diagnosis of Non-small Cell Lung Cancer Lymph Node Micrometastasis

作　　者：陈文学[1] 方选妹 黎文婷[1] 齐淑轶[1] 邹学森[1] 

机构地区：[1]江西省肿瘤医院,330029 [2]南昌市第九医院,330022

出　　处：《实用癌症杂志》2013年第6期587-589,共3页The Practical Journal of Cancer

基　　金：江西省卫生厅科技计划(20081115)

摘　　要：目的探讨SFTPB、TACSTD1、PVA基因联合检测预测非小细胞肺癌(NSCLC)微转移的可行性。方法采用荧光定量逆转录聚合酶链反应(RT-PCR)检测20例NSCLC的肺癌组织及有转移淋巴结标本中SFTPB、TACSTD1、PVA mRNA表达,20例肺良性病变的淋巴结作为阴性对照。结果 SFTPB、TACSTD1、PVA mRNA在肿瘤组织和阳性淋巴结中的表达水平相近,与良性淋巴结有显著性差异。ROC曲线分析在100.0%特异性时诊断NSCLC总的敏感性分别为SFTPB(45.7%)、TACSTD1(100.0%)、PVA(75.4%),在不同的组织学类型中PVA对鳞癌诊断的敏感性达100.0%,SFTPB对腺癌诊断的敏感性达83.2%。结论 SFTPB、TACSTD1、PVA mRNA可作为检测非小细胞肺癌患者淋巴结微转移的分子标志物,有助于早期诊断肺癌转移及组织学分型。Objective To study the feasibility of combined detection of SFTPB,TACSTD1 and PVA gene in the diagnosis of non-small cell lung cancer（NSCLC） lymph node micrometastasis. Methods The expression of SFTPB,TACSTD1 and PVA mRNA in 20 cases of NSCLC tissues and metastatic lymph node tissues were detected by Fluorescence quantitative reverse transcription polymerase chain reaction（FQ-RT-PCR） ,20 cases of benign lung lesions in lymph node were served as negative control. Results The expression of SFTPB,TACSTD1 and PVA mRNA in NSCLC tissues and positive lymph nodes was similar, there was significant differences compared with benign lung lesions in lymph node （P 〈 0.05 ）. The sensitivity of SFTPB,TACSTD1 and PVA were 45.7%, 100.0% ,75.4% respectively analyzed by ROC analysis when the specificity was 100.0%. The sensitivity of PVA for squamous cell carcinoma was 100.0%, the sensitivity of SFTPB for adenocarcinoma was 83.2%. Conclusion SFTPB, TACSTD1 and PVA mRNA are efficient indicating factors in detection of non-small cell lung cancer lymph node micrometastasis. It contributes to early diagnosis of metastasis of lung cancer and histological classification.

关 键 词：非小细胞肺癌 微转移 实时荧光定量逆转录聚合酶链反应 SFTPB基因 TACSTD1基因 PVA基因 

分 类 号：R734.2[医药卫生—肿瘤]