石杉碱甲对电休克模型大鼠海马磷酸化细胞外调节蛋白激酶及活性调节的细胞骨架联合基因活性的影响  

Effects of Huperzine A on ECT-induced changes in P-ERK and ARC of rats

作　　者：成敬[1] 王高华[1] 程自立[2] 肖玲[2] 

机构地区：[1]武汉大学人民医院精神科,430060 [2]武汉大学神经精神研究所

出　　处：《中华临床医师杂志（电子版）》2013年第16期84-87,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的观察石杉碱甲对电休克模型大鼠海马磷酸化细胞外调节蛋白激酶（P-ERK）、活性调节的细胞骨架联合基因（ARC）活性的影响。方法大鼠随机分为假电休克对照组和电休克组，再随机分为生理盐水对照组（CS组、ES组）和石杉碱甲组（CH组、EH组）。第1～17天行生理盐水或石杉碱甲灌胃；第8～17天给予假电痉挛刺激或电痉挛刺激；第18天行水迷宫定位航行实验；然后各组大鼠随机取6只处死取海马用Western blot法检测P-ERK蛋白、ARC活性。结果CS组与CH组的潜伏期无显著性差异（P〉0．05）。ES组的潜伏期显著长于CS组（P〈0．01）。EH组潜伏期与CH组无显著性差异（P〉0．05），ES组潜伏期长于EH组（P〈0．05）。CS组与CH组的P-ERK／2、ARC蛋白表达水平达水平无显著性差异（P〉0．05）。ES组的P-ERK1／2、ARC蛋白表达水平显著低于CS组（P〈0．01）。EH组P-ERK1／2、ARC蛋白表达水平与CH组无显著性差异（P〉0．05）；ES组P-ERK1／2、ARC蛋白表达水平低于EH组，差异有显著性（P〈0．05）。结论石杉碱甲能减轻电休克模型大鼠记忆损害，其机制可能与海马P-ERK、ARC的活性增加有关。Objective To investigate effects of Huperzine A on Electroshock therapy （ECT） induced changes in phosphorylated extracellular-signal related ldnase （P-ERK） and activity-regulated cytoskeletal-associated gene （ARC）. Methods Adult, male Sprague-Dawley rats were randomized to receive Huperzine A or normal saline （control）. These treatments were administered during experiment day 1-17. During experiment day 8-17, true or sham ECT were administered. At the experiment day 18 the rats were trained and tested in Morris water maze. Then six rats of each group were randomly killed to take the hippocampus for detecting P-ERK and ARC expression 1 hour after training. Results There was no significant difference in latency between CS group and CH group （P〉0.05）. The latency of ES group was significantly longer than CS group （P〈0.01）. There was no significant difference in latency between EH group and CH group （P〉0.05）. The latency of ES group was significantly longer than EH group （P〈0.05）. There was no significant difference in P - ERK1/2 and ARC protein expression level between CS group and CH group（P〉0.05）. Both the P-ERK1/2 and the ARC protein expression of ES group were significantly lower than CS group （P〈0.0!）. There was no significant difference in P-ERK1/2 and ARC protein expression level between Eli group and CH group （P〉0.05）. Both the P-ERK1/2 and the ARC protein expression of ES group were significantly lower than EH group （P〈0.01）. Conclusions Huperzine A administered before the ECT may attenuate ECT-induced amnesia. Huperzine A can reduce the effect of ECT on the expression of P-ERK and ARC. P-ERK and ARC may play a role in the development of spatial memory impairment caused by ECT.

关 键 词：电休克 石杉碱甲 细胞外调节蛋白激酶 活性调节的细胞骨架联合基因 

分 类 号：R749[医药卫生—神经病学与精神病学]