实时荧光定量PCR检测鼻咽癌组织骨桥蛋白mRNA的表达  

The mRNA expression of osteopontin in Nasopharyngeal carcinoma tissues by quantitative real-time fluorescence PCR

作　　者：韩亚琴[1] 宣世海[1] 练向阳[1] 

机构地区：[1]南通大学附属东台医院检验科,江苏东台224200

出　　处：《国际检验医学杂志》2013年第22期2949-2950,2953,共3页International Journal of Laboratory Medicine

基　　金：南通大学自然科学类研究项目(10Z093);盐城市医学科技发展计划(YK2010051)

摘　　要：目的通过检测鼻咽癌(NPC)组织的OPN的表达,探讨其表达和NPC临床病理特征的关系。方法采用实时荧光定量PCR(real-time PCR)检测NPC组织的OPN mRNA,并分析OPN的表达和NPC临床病理特征的相关性。结果 NPC组织中OPN mRNA的表达显著高于鼻咽部炎症组织(P<0.05),OPN mRNA的表达水平和NPC的T期、N期及临床分期相关(P<0.05)。结论本研究结果显示,OPN过量表达和NPC的转移和进展有关,OPN可以作为NPC个体化治疗中一个有效的预后标志物。Objective To detect the expression of OPN in Nasopharyngeal carcinoma （NPC）, and to evaluate the correlation be- tween OPN expression and clinicopathological factors. Methods Real-time polymerase chain reaction （real-time PCR） assay was performed to detect the expression of OPN in NPC tissue samples. The correlation between the OPN expression and clinicopatho- logical factors was evaluated. Results In NPC tissues, the expression levels of OPN mRNA was significantly higher than those in the nasopharyngeal inflammation tissues （P〈0.05）, and the expression level were significantly correlated with T, N and clinical stage（P〈0.05）. Conclusion All these findings suggest that overexpression of OPN is closely associated with metastasis and pro- gression of NPC. Therefore, we can speculate that OPN could be effective prognostic markers in the future for individualized treat- ment of patients with NPC.

关 键 词：骨桥蛋白 鼻咽癌 聚合酶链反应 

分 类 号：R739.63[医药卫生—肿瘤]