多参数流式细胞术在非霍奇金淋巴瘤诊断中的应用及价值  

The application and value of multiparameter flow cytometry in the diagnosis of malignant lymphoma

作　　者：易思华[1] 张晓薇[2] 赵丽[2] 周兰霞[2] 柴晓静[2] 张晨[3] 

机构地区：[1]兰州大学第一医院输血科,甘肃兰州730000 [2]兰州大学第一医院中心实验室,甘肃兰州730000 [3]兰州大学第一医院检验科,甘肃兰州730000

出　　处：《国际检验医学杂志》2013年第22期2982-2983,共2页International Journal of Laboratory Medicine

基　　金：甘肃省自然科学基金计划项目(1010RJZA084)

摘　　要：目的探讨流式细胞术(FCM)在非霍奇金淋巴瘤诊断中的价值。方法收集34例淋巴增殖性疾病的患者淋巴结穿刺液及淋巴结组织,对穿刺液进行多参数流式细胞免疫表型分析,对淋巴结组织进行病理学常规和免疫组化检查。结果 34例患者中,成熟B细胞淋巴瘤23例,19例慢性淋巴细胞白血病/小淋巴细胞淋巴瘤CD19和CD5共同表达率为100.0%。2种方法诊断非霍奇金淋巴瘤的符合率为80.6%,通过FCM免疫表型分析和病理检查,诊断恶性淋巴瘤准确率可达96.8%。结论流式细胞术对淋巴肿瘤细胞的免疫表型分析具有较高准确率,能为临床诊断非霍奇金淋巴瘤提供客观依据,具有较高的应用价值。Objective To discuss the value of multidimensional flow cytometry in the diagnosis of malignant lymphoma. Methods l.ymphnode puncture and lymphnode tissues of 34 cases of lymphoproliferative disease were collected. The lymphnode puncture were analyzed by muhiparameter flow cell immunophenotype analysis,and the lymphnode tissue were examined by the methods of pathematology and immunohistochemistry. Results There were 23 cases of mature B-cell lymphoma of 34 cases of lymphoprolifera tire disease. The expression proportion of CD19 and CD5 of 19 cases of chronic lymphocytic leukemia/small lymphocytic lymphoma were 100.0%. The match ratio of non-Hodgkin lymphoma diagnosis were 80.6% ,and the ratio could improve to 96.8% through the method of FCM pathematology and immunophenotype analysis. Conclusion The flow cytometry are more effective than other methods in the immunophenotype analysis of lymphoma cell and the former has higher accuracy rate. The flow cytometry has high clinical value and could provide objective foundation to the diagnosis of malignant lymphoma.

关 键 词：流式细胞术 非霍奇金淋巴瘤 免疫表型 

分 类 号：R733.1[医药卫生—肿瘤]