联合运用G显带核型分析技术和MLPA对100例流产胚胎进行检测  

Joint detection of G banding karyotype analysis technology and MLPA in 100 cases of abortion embryo

作　　者：刘思平[1] 刘妮[2] 熊丽[1] 邓康[1] 吴瑞枫[1] 宋兰林[1] 

机构地区：[1]南方医科大学南方医院产前诊断与遗传病诊断技术中心,广东广州510515 [2]中山大学附属第一医院病理科,广东广州510080

出　　处：《国际检验医学杂志》2013年第22期2984-2985,共2页International Journal of Laboratory Medicine

摘　　要：目的探索一种适用于临床对流产胚胎进行检测的方法。方法对100例流产胚胎绒毛组织联合应用多重连接扩增技术(MLPA)和G显带核型分析技术进行检测。结果 100例自然流产胚胎中,1例培养失败,培养成功率为99%,核型分析异常检出率为52%。MLPA对100例流产胚胎样本都获得检测结果,异常核型检出率为51%。联合应用G显带核型分析技术与MLPA后,异常核型的检出率为56%,检测灵敏度从G显带核型分析的0.893、MLPA的0.911提升到0.990。结论联合运用G显带核型分析技术与MLPA能有效地提高异常核型的检出率与检测的灵敏度,表明该方法是适合临床对流产胚胎进行检测的一种综合性方法。Objective To explore a comprehensive detection method of abortion embryo for clinical application. Methods 100 cases of abortion embryo chorionic villus were selected. Each case was divided into two parts,one for G banding karyotype, the other one for multiplex ligation dependent probe amplification（MLPA）. Results Of the 100 cases,chorionic villus was successfully cultured in 99 cases（99 %）, and abnormal embryo karyotypes were identified in 52 cases（52 %）. Chorionic villus was successfully detected in 100 cases（100%） with MLPA, and abnormal embryo karyotypes were identified in 51 cases（51%）. Abnormal embryo karyotypes were identified in 56 cases（56%） with the combined use of G banding karyotype analysis technology and MLPA at the same time. The sensitivity of joint detection was up to 0. 990,while the sensitivity of G banding karyotype analysis technology was 0. 893 ,and the sensitivity of MLPA was 0. 911. Conclusion .Joint detection of G banding karyotype analysis technology and MLPA can eKectively improve the abnormal karyotype detection rate and sensitivity, and it is a suitable and comprehensive method for clin- ical detection of aborted embryos.

关 键 词：G显带核型分析技术 多重连接扩增技术 流产胚胎 

分 类 号：R440[医药卫生—诊断学]