检测时间对速率法检测恶性肿瘤特异性生长因子结果的影响  

The influence of different detection time on TSGF detection through rate method

作　　者：文静 任传路[2] 丁庆莉[2] 

机构地区：[1]淀山湖人民医院检验科,江苏昆山215345 [2]中国人民解放军第一〇〇医院检验科,江苏苏州215007

出　　处：《国际检验医学杂志》2013年第22期3001-3002,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨采血后不同检测时间对采用速率法检测恶性肿瘤特异性生长因子(TSGF)结果的影响。方法采用比色法和速率法分别对85例恶性肿瘤患者和90例健康者于外周血血清分离4h内、12h、24h、48h、72h分别进行TSGF检测,同时采用速率法对-20℃保存状态下2组血清不同时间复融后TSGF进行检测。结果分离外周血血清4h内,恶性肿瘤组和健康对照组相比,TSGF明显升高(P<0.05);而采用2种不同方法检测时结果差异不明显(P>0.05)。4℃保存状态下,随着时间的推移,TSGF值呈现明显的下降趋势,尤其在24h后采用速率法检测,其值与4h内检测差异显著(P<0.01)。比色法下降不明显,仅在72h检测时发现有明显下降(P<0.05)。保存于-20℃时,TSGF值相对稳定,存放1周后检测结果仍差异不大(P>0.05)。结论采用全自动生化仪速率法检测TSGF时,标本应尽量在分离血清后4h内完成检测,或置于-20℃冰箱冷冻保存,否则可能会出现TSGF检测值显著下降而偏离真实结果的情况。Objective To explore the influence of different detection time on TSGF detection through rate method. Methods The blood serum samples of 85 malignant tumors patients and 90 cases of healthy controls were collected to determine the content of TSGF in 4,12,24,48 and 72 h respectively through biochemical colorimetry and rate method. They had also been determined af- ter thawing from -20 ℃. Results The detection results of TSGF in malignant tumors were higher than those of healthy controls in 4 h（P〈0.05）. And there was no difference between these two methods （P〈0.05）. With the prolongation of time, it appeared obvious downward trend through rate method,especially after 24 h（P〈0.01）. But this phenomenon was not obvious in TSGF de- tection through the method of biochemical colorimetry（P〉0.05） which only observed at 72 h（P〈0.05）. The detection results of TSGF were relatively stable when preserved in -20 ℃ （P〈0.05）. Conclusion Through rate method, detection of TSGF should be finished in 4 h and as soon as possible,or samples should be preserved in -20 ℃ ,otherwise the results will decline with the prolon- gation of time. Key words： rate method 〈 biochemical analyzer ; TSGF; detection time

关 键 词：速率法 生化仪 恶性肿瘤特异性生长因子 检测时间 

分 类 号：R730.4[医药卫生—肿瘤]