白藜芦醇对衰老模型小鼠脑组织形态学和氧化应激水平的影响  

Effects of resveratrol on morphology and oxidative stress of brain tissues in aging mice

作　　者：崔玉丰[1] 张博妍 张瑞[1] 李翠[1] 赵越[1] 任亚浩[1] 杨军[1] 

机构地区：[1]中国医科大学公共卫生学院营养与食品卫生教研室,沈阳110001

出　　处：《卫生研究》2013年第6期995-998,1003,共5页Journal of Hygiene Research

摘　　要：目的探讨白藜芦醇对衰老小鼠脑组织的保护作用。方法 54只16周龄雄性昆明小鼠随机分为对照组、衰老组、干预组。对照组皮下注射生理盐水,其余两组皮下注射200 mg/kg BW的D-半乳糖制备衰老小鼠模型,干预组给予22.5 mg/kg BW的白藜芦醇灌胃,其余两组给予0.5%的羧甲基纤维素钠溶液灌胃。干预16周后处死动物,观察小鼠脑组织形态结构,脏器指数,谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、单胺氧化酶(MAO)活性以及丙二醛(MDA)含量的变化。结果与对照组相比,衰老组小鼠神经细胞数量减少且发生变性(P<0.05),脏器指数低于对照组(P<0.05),脑组织GSH-Px、SOD及CAT活性均降低(P<0.05),MAO活性及MDA含量均增高(P<0.05)。与衰老组相比,干预组小鼠神经细胞数量增多(P<0.05),细胞形态明显改善,脏器指数高于衰老组(P<0.05),脑组织GSH-Px、SOD及CAT活性均增高(P<0.05),MAO活性及MDA含量均降低(P<0.05)。结论白藜芦醇能维持衰老小鼠神经细胞正常形态结构,降低其氧化应激水平,对衰老模型小鼠脑组织具有保护作用。Objective To investigate the protective effects of resveratrol on brain tissues in aging mice induced by D-galaetose. Methods 54 Kunming male mice aged 16 weeks were randomly divided into control group, aging group, intervention group. The control group was prepared by subcutaneous injection of normal saline and the other two groups were prepared by subcutaneous injection of D-galactose （200 mg/kg BW）. The intervention group was fed with resveratrol（22.5 mg/kg BW）by oral gavage and the other two groups were fed with solution of 0.5 % sodium eetrboxymethyl cellulose. All animals were sacrifieed 16 weeks later, morphology of brain was observed, calculate the organ eoeffieients, and GSH-Px, SOD, CAT, MAO activity and MDA content were detected in the mice brain. Results Compared with the control group, the aging group lost normal morphological structure of nerve cells and the number of nerve cells was significantly decreased （ P 〈 0.05 ） , the organ coefficients were significantly decreased （ P 〈 0. 05 ） , and GSH-Px, SOD, CAT activities were significantly decreased （ P 〈 0.05 ） , MAO activity andMDA content were significantly increased in brain of the aging group （P 〈 0.05 ）. Compared with the aging group, the intervention group brain maintain normal morphological structure of nerve cells and the number of nerve cells was significantly increased （ P 〈 0.05 ）, the organ coefficients were significantly increased （ P 〈 0.05 ） , and GSH-Px, SOD, CAT activities were significantly increased （ P 〈 0.05 ）, MAO activity and MDA content were significantly decreased in brain of the intervention group （ P 〈 0.05 ）. Conclusion Resveratrol can maintain normal morphological structure of nerve cells of aging mice ,decrease oxidative stress responses, and has protective effects on brain tissues in aging mice induced by D-galactose.

关 键 词：白藜芦醇 D-半乳糖 衰老 氧化应激 

分 类 号：R339.38[医药卫生—人体生理学] Q593.2[医药卫生—基础医学]