微波辐射对人胰腺癌JF305细胞增殖的抑制作用及其机制  

Inhibitory effect of microwave radiation on proliferation of human pancreatic cancer JF305 cells and its mechanism

作　　者：朱文赫[1] 张巍[1] 李妍[1] 徐俊杰[1] 罗军[1] 姜艳霞[1] 芦晓晶[1] 吕士杰 

机构地区：[1]吉林医药学院生物化学与分子生物学教研室,吉林132013

出　　处：《卫生研究》2013年第6期1008-1011,共4页Journal of Hygiene Research

基　　金：吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2013]第479);吉林省科技发展计划项目合同书(No.20120941)

摘　　要：目的探讨不同强度,频率为2450 MHz微波辐射对人胰腺癌JF305细胞增殖的抑制作用及其机制。方法分别以强度为2.5、5.0、10.0、15.0和20.0 mW/cm2的微波处理JF305细胞20 min,24 h后采用MTT法检测微波辐射后对细胞增殖的抑制情况,Annexin V-FITC和PI双染检测细胞凋亡,Caspase-3活性检测试剂盒检测辐射后细胞Caspase-3活性;Western blotting法检测细胞内Caspase-3和HSP 70蛋白表达量。结果微波辐射作用JF305细胞后,细胞增殖活性明显受到抑制,细胞发生凋亡,随着辐射强度的提高,细胞凋亡率明显增加。随着辐射强度的增加,细胞内Caspase-3活性同对照组细胞相比呈上升趋势,差异具有统计学意义;细胞内Caspase-3和HSP 70蛋白表达水平也随着辐射强度的增加呈上升趋势。结论微波辐射对人胰腺癌JF305细胞增殖具有抑制作用,其可能机制是通过改变细胞内的应激水平而引起细胞凋亡。Objective To investigate on the proliferation effect of different intensities 2450 MHz microwave radiation on human pancreatic cancer JF305 ceils and its possible mechanism. Methods JF305 cells were radiated by intensity of 2.5,5.0,10.0,15.0 and 20.0 mW/cm2 microwave for 20 rain. The proliferation capacity of JF305 was measured by MTT assays, Annexin V-FITC and PI staining was used for detecting cell apoptosis. The activity of Caspase-3 was examined. The expressions of Caspase-3 and HSP 70 protein after the cell treatment with microwave were detected by Western blotting. Results After microwave radiation, the proliferation inhibition rates of JF305 cells were significantly higher compared with control group. Annexin V-FITC and PI staining result showed that microwave radiation could induce cell apoptosis. Caspase-3 increased after radiated by microwave, compared with control group（P 〈 0. 05）. Results of Western blotting showed that the expression of Caspase-3 and HSP 70 protein increased significantly in differentdosage radiation group. Conclusion Microwave JF305 cells, the possible mechanism may be changing of stress level. radiation can inhibit related with inducing the proliferation of cell apoptosis by

关 键 词：微波辐射 细胞增殖 细胞凋亡 热应激蛋白70 CASPASE-3 

分 类 号：R735.9[医药卫生—肿瘤] R818.74[医药卫生—临床医学]