PCR-反相杂交法检测沙门氏菌invA、invE基因  

Detection of invA and invE gene of bacterium typhosum by PCR-reversed hybridization

作　　者：冯同喜[1] 王燕宁[1] 张建芳[2] 李绣萍[1] 卢媛[1] 李建平 苏明权[2] 

机构地区：[1]兰州军区兰州总医院,甘肃兰州730050 [2]第四军医大学西京医院

出　　处：《西北国防医学杂志》2000年第4期303-305,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的 :探讨从血液中早期检出伤寒沙门氏菌病原体的方法。方法 :选择沙门氏菌属中的invAinvE ,采用PCR扩增 -反相杂交法及电泳法同时对血培养物中的伤寒沙门氏菌进行对比检测。结果 :电泳法 6h检出 8份 ,检出率 80 %。 8h、10h可将 10份全部检出 ,检出率为 10 0 %。反相杂交法 4h有 3份阳性 ,阳性率为 30 % ,6h有 9份出现阳性反应 ,阳性率为 90 % ,8h、10h可将 10份全部检出 ,阳性率为 10 0 %。临床标本中电泳法 12 / 16阳性 ,阳性率 75 % ,PCR -反相杂交法阳性 14/ 16 ,阳性率87.5 %。结论 :反相杂交法具有敏感性高、特异性好等优点 ,对伤寒的早期快速诊断及治疗有重要意义。Objective:[WT5BZ]To explore the methods to detect bacterium typhosum of blood in early stage.[WT5HZ]Methods:[WT5BZ]InvA and invE gene of salmonelleae in blood culture material were examined by PCR-reversed hybridization and electrophoresis method.[WT5HZ]Results:[WT5BZ]Of 8(80%)and 10(100%) samples could be detected positively by agarose electrophoresis method respectively after 6 h and after 8 h and 10 h. However,3(30%) samples were detected positively by reversed hybridization after 4 h and 9(90%) and 10(100%)samples were positive after 6 h and 8 h.The positive findings of clinical specimen were 12/16(75%)and 14/16(87.5%)respectively by agarose electrophoresis and by PCR-reversed hybridization method.[WT5HZ]Conclusion:[WT5BZ]The results show that PCR-reversed hybridization method is obviously superior to agarose electrophoresis for the detecting of invA and invE gene.The method has high sensibility.specificity and reliability and has certain significance for the early diagnosis and treatment of typhoid. [WT5HZ]

关 键 词：伤寒沙门氏菌 聚合酶链反应 反相杂交 inv基因 

分 类 号：R516.304[医药卫生—内科学]