左旋多巴对体外培养大鼠纹状体的神经毒性及其保护方法的研究  

Study on the neurotoxicity of L-dopa in cultured rat striatal neurons and its neuroprotective method

作　　者：周国庆[1] 陈光辉[1] 周孝达[2] 

机构地区：[1]南京军区南京总医院神经内科,江苏南京210002 [2]上海第二医科大学附属仁济医院,上海200010

出　　处：《中风与神经疾病杂志》2000年第6期331-332,共2页Journal of Apoplexy and Nervous Diseases

摘　　要：目的　研究左旋多巴 (L- dopa)的神经毒性及其神经保护方法。方法　用胎盼兰染色法检测 L- dopa和抗氧化剂还原型谷胱甘肽 (GSH)、NMDA受体拮抗剂 MK80 1对体外培养大鼠纹状体的神经元活力的影响。结果　 L- dopa使大鼠纹状体神经元活力明显下降 (P<0 .0 1) ,GSH和 MK80 1可明显减轻 L- dopa引起的纹状体神经元活力下降 (P<0 .0 1)。结论　 L- dopa可能通过氧化应激和兴奋 NMDA受体两个途径发挥其神经毒性。抗氧化剂或 NMDA受体拮抗剂与 L- dopa合用可能减轻 L- dopa的神经毒性。Objective To study the neurotoxicity of L dopa and its neuroprotective method. Method The effect of L dopa and antioxidant reduced glutathione (GSH) as well as NMDA receptor antagonist MK801 on cell viability of cultured rat striatal neurons was assessed by the trypan blue exclusion method. Results L dopa (200μM) resulted in decrease in cell viability of striatal neurons (P<0.01),GSH (10mM) and MK801 (100μM) might significantly antagonize L dopa induced decrease in cell viability of striatal neurons (P<0.01). Conclusion L dopa seems to induce cytotoxicity on neurons via two different pathways,one is oxidative stree mechanism,the other is mediated by NMDA receptor. A combination of L dopa and NMDA receptor antagonists may decrease the neurotoxicity of L dopa.

关 键 词：左旋多巴 神经毒性 帕金森病 纹状体 大鼠 

分 类 号：R742.505[医药卫生—神经病学与精神病学]