咬合力丧失影响大鼠牙周组织白细胞介素-6变化的研究  

Effects of Bite Force Lost on the Expression of IL-6 in the Rat Periodontium

作　　者：袁林[1] 赵云凤[1] 周伟东[2] 

机构地区：[1]华西医科大学口腔医院口腔修复科,610041 [2]第一军医大学珠江医院

出　　处：《华西口腔医学杂志》2000年第6期367-368,400,共3页West China Journal of Stomatology

摘　　要：目的 :探讨咬合力正常及丧失状态下 ,大鼠牙周细胞IL 6的动态表达 ,初探IL 6在牙周组织改建过程中的分子机理。方法 :选用Wistar大鼠建立正常咬合力、咬合力丧失的动物模型 ,采用HE染色和免疫组化的方法 ,应用MIAS 2 0 0 0图像分析仪 ,对实验组和对照组各时间点牙周膜染色强度进行测量 ,观察其牙周形态变化以及牙周组织中IL 6蛋白表达的动态变化。结果 :咬合力丧失引起牙周细胞中IL 6表达较正常咬合力时明显增强 ,同时观察到牙周膜结构紊乱、牙槽骨吸收。结论 :咬合力丧失促使牙周组织产生IL 6明显增多且呈规律性变化 ,提示IL 6在咬合力影响牙周组织改建的过程中起着重要的调节作用。Objective: In order to explain the molecular mechanism of periodontium remodeling the expression of IL 6 in the rat periodontal ligament fibroblasts (PDLC) and the alveolar bone osteoblasts under normal and bite force lost was studied. Methods: To establish a rat model of different bite force for the study, left maxillary molars of rats were extracted and the left mandibular molar area was used as the bite force lost. The animals were sacrificed after 6 hours, 1, 2, 3 days and 1, 2, 3, 4 weeks. The prepared tissue specimens were processed for the study of the change in the histologic morphology and the expression of IL 6 protein in PDLC and osteoblasts with HE staining and immunohistochemistry techniques. Results: In the group of bite force lost, the loosely arrangement of periodontal ligaments and the resorption of alveolar bone were observed in histological study, the expression of IL 6 in PDLC and osteoblasts was enhanced apparently than that in the group of normal bite force by using immunohistochemistry techniques. In the group of lost bite force, the expression of IL 6 in PDLC enhanced with time regularly: The expression of IL 6 was strengthened after 3 days, and reached the peak after 2 weeks, then, decreased after that and restored to normality in 4 weeks. While in osteoblasts of the group of bite force lost, the expression of IL 6 was strengthened just after 2 weeks. Conclusion: Bite force lost induce the expression of IL 6 enhanced apparently in PDLC and osteoblasts, it suggests that IL 6 may play important roles in the effect of bite force on periodontium remodeling. It approaches initially the molecular mechanism of periodontium remodeling, demonstrates that the histologic morphology is closely related to the mechanical condition. \;

关 键 词：咬合力 白细胞介素-6 牙周组织改建 免疫组化 

分 类 号：R780.2[医药卫生—口腔医学]