分泌型卷曲相关蛋白1与慢性牙周炎的相关分析  

Exploring the relationship between secreted frizzled-related protein 1 and chronic periodontitis

作　　者：袁海波[1] 金晶[1] 许春姣[1] 邱喜丽 王璐[2] 吕逢源[2] 刘婷婷[2] 吴颖芳[1] 尹晓敏[1] 方厂云[1] 阙国鹰[1] 彭解英[1] 

机构地区：[1]中南大学湘雅医院口腔科,长沙410008 [2]中南大学口腔医学院牙周黏膜病学教研室,长沙410078

出　　处：《华西口腔医学杂志》2013年第6期615-618,共4页West China Journal of Stomatology

基　　金：湖南省科技厅基金资助项目(2011SK3254)

摘　　要：目的检测分泌型卷曲相关蛋白1(SFRP1)在慢性牙周炎(CP)患者和牙周健康者的表达情况,探讨SFRP1在CP的发生与发展过程中的作用。方法选择CP患者28例为试验组,按临床附着丧失(CAL)程度分为轻、中、重度3组,另外选择牙周健康者10例为对照组。收集两组研究对象的龈沟液,采用酶联免疫吸附法测定龈沟液中SFRP1的质量浓度。取试验组中22例CP患者的病变牙龈组织标本,以及10例对照组的正常牙龈组织,进行SFRP1免疫组织化学染色,观察着色强度,分析SFRP1表达与不同程度CP的相关性。结果 1)试验组和对照组龈沟液中SFRP1的质量浓度分别为(281.07±33.37)和(245.30±35.69)ng·L-1,试验组明显高于对照组(P<0.05);龈沟液内SFRP1质量浓度与CAL呈明显正相关(r=0.651,P<0.001)。2)试验组SFRP1着色强度积分值(4.500±0.913)明显高于对照组(2.800±1.135)(P<0.001);试验组中,轻、中、重度CP组间的着色强度积分值无明显差异(P>0.05)。结论SFRP1在CP患者龈沟液及牙龈组织中的表达高于牙周健康者,SFRP1可能参与了CP的发生发展过程。Objective This study detects the expression of secreted frizzled-related protein 1 （SFRP1） in healthy patients and patients with chronic periodontitis （CP） and explores the relationship between SFRP 1 and the occurrence and development of CP. Methods First, 28 patients forming the CP group were further divided into mild, moderate, and severe CP subgroups according to clinical attachment loss （CAL） data. Ten healthy volunteers were recruited in the control group. Gingival crevicular fluid （GCF） was collected from all of the patients, and the concentration of SFRP 1 in the GCF samples was detected using enzyme-linked imrnunosorbent assay. Next, gingival lesions were obtained from 22 patients in the CP group and healthy gingival tissues were obtained from the 10 healthy patients in the control group. Immunohistochemical analysis for SFRP1 was used to analyze the correlation between the expression of SFRP1 and the severity of CP based on staining intensities. Results The concentration of SFRP1 in GCF samples taken from of the CP group （281.07 ng.L-1±33.37 ng·L-1） was significantly higher than that in samples taken from the control group （245.30 ng.L-1±35.69 ng.L-1） （P〈0.05）. A significant positive correlation was observed between the concentration of SFRP1 in GCF and CAL （r=0.651, P〈0.001）. Furthermore, the SFRP1 scores in the CP groups （4.500±0.913） were significantly higher than those in the control group （2.800±1.135） （P〈0.001）. SFRP1 scores did not vary significantly among the CP subgroups （P〉0.05）. Conclusion SFRP1 expression in the CP groups was significantly higher than that in the control group. Thus, SFRP1 may play a significant role in the development of CP.

关 键 词：慢性牙周炎 龈沟液 分泌型卷曲相关蛋白1 

分 类 号：R781.4[医药卫生—口腔医学]