不同龋敏感儿童菌斑中变异链球菌数量及菌群比例的定量分析  

Quantitative analysis of Streptococcus mutans and its proportion in the dental plaque of different caries-susceptible children

作　　者：赵河川[1,2] 陈霄迟[2,3] 徐韬[2] 

机构地区：[1]重庆医科大学附属口腔医院预防科·口腔疾病与生物医学重庆市重点实验室,重庆400015 [2]北京大学口腔医学院·口腔医院预防教研室,北京100081 [3]北京大学口腔医学院·口腔医院生物教研室,北京100081

出　　处：《华西口腔医学杂志》2013年第6期619-623,共5页West China Journal of Stomatology

摘　　要：目的比较不同龋敏感儿童口腔菌斑中变异链球菌数量及其在菌群中比例的差异。方法采集26名3～4岁不同龋敏感的儿童牙面菌斑，运用TaqMan探针实时荧光定量聚合酶链反应（PCR）检测有龋组和无龋组儿童菌斑中变异链球菌和总菌数量，以及变异链球菌在总菌群中所占的比例，并对结果进行分析比较。结果有龋组和无龋组儿童每毫克菌斑中变异链球菌菌落数分别为1．33×10^5、1．16×10^3CFU·mg^-1，二者间差异有统计学意义（P=-0．033）；每毫克干重菌斑中总菌落数分别为7．17×10^7、1．01×10^8CFU·mg^-1，二者间差异无统计学意义（P=-0．418）：有龋组和无龋组变异链球菌在总菌中所占比例分别为0．0586和0．0186，二者间差异有统计学意义（P=-0．008）。结论无龋与患龋儿童牙面总菌群数量差异无显著性，但患龋儿童牙面菌斑中变异链球菌数量更多，在总菌群中所占比例更大。提示菌斑中变异链球菌与总菌的比例与儿童患龋风险密切相关，可以作为评估龋易感性和预测龋病发展趋势的新指标。Objective To determine the quantity of Streptococcus mutans （S. mutans） and the ratio ofS. mutans to total bacteria in the dental plaque of different caries-susceptible children. Methods Dental plaque samples from 26 children aged 3 years old to 4 years old were collected. The quantities of S. mutans and total bacteria were determined by TaqMan real-time fluorescence quantitative polymerase chain reaction （PCR）. The ratio ofS. mutans to total bacteria in children with and without caries was calculated and statistically analyzed. Results In children with and without caries, the quantities ofS. mutans were 1.33±105 and 1.16±10^3 CFU·mg^-1, the total bacteria were 7.17±107 and 1.01±108 CFU·mg^-1, and the ratios ofS. mutans to total bacteria were 0.058 6 and 0.018 6, respectively. Significant differences were observed in the quantities of S. mutans and the total bacteria as well as in the ratios ofS. mutans to total bacteria of the two groups （P=0.033, 0.418, 0.008）. Conclusion The quantities of total bacteria of the two groups show negligible difference. However, the quantity of S. mutans and the ratio ofS. mutans to total bacteria in caries-susceptible children are higher than those in caries-free children. Therefore, the ratios of S. mutans to total bacteria in plaque are closely associated with the prevalence of dental caries in children.

关 键 词：龋齿 乳牙 牙菌斑 变异链球菌 实时荧光定量聚合酶链反应 

分 类 号：R781.1[医药卫生—口腔医学]