甘草酸二铵联用红花注射液对大鼠溃疡性结肠炎炎症的影响  

Therapeutic effects of diammonium glycyrrhizinate combined with safflower solution on ulcerative colitis in rats

作　　者：原皓[1] 王鹤鸣[1] 韩雪[1] 

机构地区：[1]潍坊医学院临床学院,山东省潍坊市261042

出　　处：《世界华人消化杂志》2013年第31期3338-3343,共6页World Chinese Journal of Digestology

基　　金：山东省自然科学基金资助项目;No.ZR2010HM089~~

摘　　要：目的:建立2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)诱导的溃疡性结肠炎(ulcerative colitis,UC)大鼠模型,对比甘草酸二铵(diammonium glycyrrhizinate,DG)联合红花注射液(safflower solution,SS)与单用DG的疗效及对促炎因子白介素-8(interleukin-8,IL-8)表达的影响,从而探讨抗炎药DG联合改善微循环的SS治疗UC的价值.方法:40只健康SD大鼠随机分为正常对照组、实验组(DG+SS)、DG组和模型组,每组10只.实验组、模型组、DG组大鼠用2,4,6-三硝基苯磺酸(TNBS)灌肠复制溃疡性结肠炎模型,实验组每天给予40 mg/kg DG加400 mg/kg SS腹腔注射干预治疗,DG组每天给予40 mg/kg DG治疗,正常对照组及模型对照组给予等容积生理盐水腹腔注射.10 d后观察大鼠疾病活动指数(disease activity index,DAI)及结肠组织学变化,并用免疫组织化学方法检测大鼠结肠IL-8的表达.结果:正常对照组、实验组(DG+SS)、模型组和DG组的DAI评分分别为1.4±0.3、2.4±0.2、3.8±0.4和7.6±0.9;组织学损伤评分分别为1.5±0.4、2.4±0.4、3.7±0.6和6.3±1.1;IL-8阳性率分别为15.7%±3.7%、20.8%±4.7%、34.7%±4.9%和56.4%±6.9%.与模型组相比,实验组与DG组的DAI和组织学表现显著改善,IL-8的表达显著降低(P<0.01),而实验组又比DG组效果好(P<0.05),经one-way ANOVA及SNK-q检验,差异有统计学意义.结论:DG联合SS对大鼠UC的疗效及对促炎因子IL-8表达的抑制作用均好于DG单独的作用,提示抗炎联合改善微循环是一种较单用抗炎药物治疗UC的更有效的方法,更有效地抑制IL-8的生成及改善微循环是其可能的机制之一.AIM： To evaluate the therapeutic effects of diam- monium glycyrrhizinate （DG） combined with safflower solution （SS） on experimental colitis and on the expression of interleukin-8 （IL-8） in rats. METHODS： Forty Sprague-Dawley rats were randomized into a normal control group, a treatment group （DG combined with SS）, a DG group and a TNBS control group, with 10 rats in each group. Colitis was induced by rectal administration of TNBS in rats in the treatment group, DG group and TNBS control group. Rats in the treatment group were treated with DG （40 mg/kg） and SS （400 mg/kg） every day, and the DG group was treated with DG （40 mg/kg） every day. Disease activity index （DAI） and his- tological damage score were calculated, and the expression of IL-8 in colon tissues was detected by immunohistochemistry. RESULTS： In the normal control group, treat- ment group, DG group and TNBS control group, DAIs were 1.4 ± 0.3, 2.4 ± 0.2, 3.8 ± 0.4 and 7.6 ± 0.9, respectively; histological damage scores were 1.5 ± 0.4, 2.4± 0.4, 3.7 ± 0.6 and 6.3 ± 1.1, re- spectively; and the positive percentages of IL-8 were 15.7% ± 3.7%, 20.8%± 4.7%, 34.7% ± 4.9% and 56.4%± 6.9%, respectively. Compared with the TNBS control group, DAI and histological injury scores were significantly improved in the treatment group and DG group, and the expres- sion of IL-8 was decreased significantly in the treatment group （P 〈 0.01 or 〈 0.05）. Compared with the DG group, DAI and histological dam- age scores were significantly improved and the expression of IL-8 was decreased significantly in the treatment group （P 〈 0.01 or 〈 0.05）. CONCLUSION： DG combined with SS exerts a better effect than DG alone in the treatment of experimental ulcerative colitis in rats, and the underlying mechanism is possibly associated with affecting the expression of IL-8.

关 键 词：甘草酸二铵 红花注射液 溃疡性结肠炎炎症 治疗 

分 类 号：R574.62[医药卫生—消化系统]