红毛五加多糖对体外人胃癌细胞增殖的抑制作用及其机理  

The Inhibitory Effect of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides on Proliferation of Gastric Cancer Cell Line and Its Mechanism

作　　者：吕晓英[1] 曾令福[1] 苏勉诚[2] 刘新华[3] 杨培全 

机构地区：[1]华西医科大学公共卫生学院营养与食品卫生学教研室,成都 610041 [2]珠海丽珠集团国家中药中心 [3]成都军区军事医学研究所

出　　处：《华西医科大学学报》2000年第4期478-480,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金部分资助项目(批准号 39070111)

摘　　要：为探索红毛五加多糖 (AGP )对胃癌细胞增殖的抑制作用及其作用机理 ,作者采用生长曲线和群体倍增时间反应癌细胞增殖情况 ,用流式细胞仪检测 AGP 对胃癌细胞癌基因蛋白表达的影响。结果显示 :AGP 能显著抑制胃癌细胞的增殖 ,且有时间 -效应关系和剂量 -效应关系。经 1.0× 10 - 4 m ol/L AGP 作用 48小时后 ,胃癌基因 c- myc蛋白表达与空白对照组比较无显著性差异 (P>0 .0 5 )。上述实验结果表明 ,AGP 对胃癌细胞的增殖有显著抑制作用 ,其抑癌机理可能与癌基因 c- myc蛋白表达无关。The purpose of this study was to investigate the inhibiting effect of Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides (AGPⅢ) on the proliferation of gastric cancer cell. and to acquire knowledge about the possible mechanism. The proliferation curve and the cell double time were adopted to reflect the proliferation of gastric cancer cell. Also, flow cytometry was adopted to explore the protein expression of the c myc oncogene of gastric cancer. The results showed that the proliferation of gastric cancer cell was significantly inhibited by AGPⅢ, and the time effect and dose effect relationships were evident. The protein expression of the c myc oncogene did not change significantly after the gastric cell was cultured with 1.0×10 -4 mol/L AGPⅢ for 48h ( P >0.05). The above results showed that AGPⅢsignificantly inhibited the proliferation of the in vitro human gastric cancer cell, and its mechanism may not have a keen relationship with the expression of the c myc oncogene.

关 键 词：红毛五加多糖 胃癌 细胞增殖 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]