皮层扩散性抑制对大鼠脑缺血后谷氨酸载体EAAC1 mRNA表达的影响  

Effect of Cortical Spreading Depression (CSD) on the Expression of Glutamate Transporter EAAC1 mRNA Following Focal Cerebral Ischemia in Rats

作　　者：张峰[1] 周良辅 黄峰平 刘艳红[2] 费俭[2] 

机构地区：[1]复旦大学医学院华山医院神经外科,200040 [2]中国科学院上海细胞生物学研究所,200031

出　　处：《中国临床神经科学》2000年第4期256-259,共4页Chinese Journal of Clinical Neurosciences

摘　　要：方法：用氯化钾诱发ＳＤ大鼠皮层扩散性抑制（ＣＳＤ），改良 Ｌｏｎｇａ法制作大鼠 ＭＣＡＯ模型，用地高辛标记的 ｃＲＮＡ探针，行原位杂交检测谷氨酸载体ＥＡＡＣ１ ｍＲＮＡ表达。结果：假手术组脑片上单位面积ＯＤ为（２ １８９±２６３），阳性颗粒数为（４３．２±４．７）个。缺血 ３０ｍｉｎ时皮层ＥＡＡＣ１ ，ｍＲＮＡ表达明显上调，经ＣＳＤ处理后再缺血时，与对照组比较表达水平均明显增高，其中Ｌｉ ３０ｍｉｎ时最高。结论：ＣＳＤ的作用可能与诱导ＥＡＡＣ１合成，增加谷氨酸的摄取有关。Methods :CSD was induced in rats with 3 mol .L-l KCl,and the modified Longa's method was used to make focal cerebral ischemia model. The expression of glutamate transporter EAACl mRNA were detected by in situ hybridization with DIG-labeled cRNA probe. Results: In sham-operated group, the OD in an area unit was 2l89 ±263,the number of positive cells was 43. 2±1. 7. Following ischemia for 30 min,the expression of EAACl mRNA in cortex was obviously up-regulated. In ex- periment group,the expression of EAACl mRNA was obviously higher than that in control group (P<0. 05). The effect of CSD is maybe inducing the synthesis of EAAC1 and increasing the uptake of glutamate. Conclusion :EAACl may play an im- portant role in the regulation of glutamate concentration following ischemia, the effect of CSD may induce the synthesis of EAAC1 and increase the uptake of glutamate.

关 键 词：脑缺血 皮层扩散抑制 谷氨酸载体 MRNA 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]