寄生取食Cry1Ab杀虫蛋白的粘虫幼虫对伞裙追寄蝇寄生率及生长发育的影响  

Performances of tachinid fly Exorista civilis (Diptera: Tachinidae) parasitizing host Mythimna separata (Lepidoptera:Noctuidae) larvae fed with Cry1Ab toxin

作　　者：杨海霞[1,2] 程云霞[1] 张蕾[1] 牛长缨[2] 罗礼智[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193 [2]华中农业大学植物科学技术学院,武汉430070

出　　处：《昆虫学报》2013年第10期1166-1173,共8页Acta Entomologica Sinica

基　　金：转基因生物新品种培育重大专项(2009ZX0809211-018B)

摘　　要：【目的】研究取食含Cry1Ab杀虫蛋白饲料的粘虫Mythimna separata(Walker)对其寄生天敌伞裙追寄蝇Exorista civilis Rondani的寄生率、生长发育和产卵量的影响。【方法】以取食含不同浓度(0,3.125,6.25,12.5和25μg/g)Cry1Ab杀虫蛋白饲料的粘虫幼虫为寄主,观察并记录伞裙追寄蝇的寄生和生长发育等指标。【结果】取食过不同浓度Cry1Ab蛋白的粘虫对伞裙追寄蝇寄生率、产卵量没有显著的影响(P≥0.05),即不会影响寄生蝇对寄主的选择。取食这5种浓度杀虫蛋白的粘虫上寄生的伞群追寄蝇发育历期(卵-幼虫、蛹、成虫产卵和世代)、羽化率、性比和产卵量均无显著差异(P≥0.05),但对蝇蛆存活率和蛹重影响显著(P<0.05)。其中,伞裙追寄蝇蝇蛆存活率以寄生在取食3.125μg/g Cry1Ab蛋白的粘虫上的最高(33%),并显著高于对照和其余3个浓度处理(P<0.05),但这4个处理之间的存活率差异不显著(P≥0.05)。伞裙追寄蝇的蛹重随寄主取食Cry1Ab杀虫蛋白含量的增加而显著下降(P<0.05)。其中对照的蛹重为49.8 mg,而寄生于取食6.25和25μg/g蛋白寄主上的蛹重分别只有41.0和36.7 mg。【结论】本实验结果表明寄主粘虫取食Cry1Ab蛋白对伞裙追寄蝇无显著负面影响,为科学评价田间转Bt基因作物对寄生蝇的影响作用提供了重要的科学依据。[ Aim] To demonstrate the effect of transgenic Bt-crop on the bionomics of the tachinid fly Exorista civilis Rondani, the parasitism rate and life history parameters of E. civilis developed from larvae of the oriental armyworm, Mythimna separata （Walker）, fed on artificial diets with Cry1Ab toxin were investigated in laboratory. [ Methods] M. separata larvae were fed on diets containing 0, 3. 125, 6.25, 12.5 and 25 μg/g of Cry1Ab toxin, respectively, and used as the host for E. civilis. The parameters related to the parasitism and the growth and development of E. civilis on the host were observed and recorded. [ Results ] The results showed that the parasitism rate and fecundity of the fly on M. separata larvae feeding on diets containing various doses of Cry1 Ab protein were insignificantly different （P ≥ 0.05 ）, suggesting that host selective behavior of the E. civilis is not affected by the dose of Bt-toxin in the caterpillar. The developmental period （egg-larva, pupa, adult oviposition and generation ）, emergence rate, sex ratio, and fecundity of E. civilis developed from M. separata larvae treated by various concentrations of Cry1Ab toxin were insignificantly different （P≥0.05）. However, the survival rate of the maggots in the host feeding on diet containing 3. 125 μg/g of greater than that of the control and the rest treatments （P 〈 0.05）, while Cry1Ab toxin was significantly insignificantly different amongthe remained 4 treatments （P ≥ 0.05 ）. The pupal weight significantly declined as the concentration of Cry1Ab toxin fed by their hosts increased （P 〈0.05）. The pupal weight in the control group was 49.8 mg, while that in treatment groups of 6.25 and 25 μg/g Cry1Ab toxin was only 41.0 and 36.7 mg, respectively. [ Conclusion ] These results indicate that parasitizing host M. separata larvae fed with Cry1 Ab toxin has no dramatically negative effect on parasitoid E. civilis. This study provides a basis for the evaluation of the risk of transgenic Bt-crops on the tachi

关 键 词：伞裙追寄蝇 粘虫 CRY1AB杀虫蛋白 寄生 生长发育 存活率 

分 类 号：Q965.9[生物学—昆虫学]