季德胜蛇药片对大鼠肺缺血再灌注损伤的保护作用  

The protective effects of Jidesheng Sheyao pian on lung ischemiareperfusion injury in rats

作　　者：陶冬英[1] 倪晶晶[1] 任典寰[1] 于纪棉[1] 张秀娟[1] 万勇[1] 

机构地区：[1]浙江宁波卫生职业技术学院人体形态教研室

出　　处：《中国临床药理学与治疗学》2013年第11期1219-1223,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省教育厅课题项目(Y201226089)

摘　　要：目的:探讨季德胜蛇药片对大鼠肺缺血再灌注损伤的保护作用及其机制。方法:将30只清洁级SD雄性大鼠分为季德胜蛇药组(D组)、对照组(C组)和缺血再灌注组(I/R组),每组10只。建立大鼠肺缺血再灌注模型,检测大鼠肺组织湿干重比;光学显微镜及透射电镜下观察肺组织损伤程度;通过明胶酶谱法测定肺组织匀浆液中明胶酶即金属蛋白酶-2、-9(MMP-2、MMP-9)的活力;逆转录酶PCR方法检测MMP-2、MMP-9mRNA在大鼠肺组织中的表达变化。结果:与缺血再灌注组比较,蛇药组肺湿干重比降低(P<0.01),肺组织损伤程度减轻,肺组织匀浆液MMP-9酶活力显著下降,MMP-2酶活力略有降低,肺组织中MMP-9、MMP-2的mRNA表达均降低。结论:季德胜蛇药片通过抑制MMP-9、MMP-2的活性及表达,使基底膜的降解降低,从而对大鼠肺缺血再灌注损伤有一定的保护作用。AIM： To investigate the protective effects and the mechanisms of Jidesheng Sheyao pian on lung ischemia-reperfusion injury of rats. METHODS： Thirty SD male rats were divided into Jidesheng Sheyao group （n = 10）, control group （n= 10） and ischemia-reperfusion injury group （I/R, n=10）. The models of lung ische-mia-reperfusion injury of SD rats were estab lished. Then, the wet/dry ratio was measured, the histopathology of the lung injury was ob served by light microscope and electron micro scope, the activity of matrix metalloproteinase-2 （MMP-2）, matrix metalloproteinase-9 （MMP- 9） in the lung homogenates were detected bY zy mography, RT-PCR the expression of MMP-2, MMP-9 mRNA were detected in rat lung tis sues. RESULTS：Compared with the I/R group,the wet/dry ratio were significantly decreased （P〈0.01）, the degree of lung injury showed lighter, the activity of MMP-9 in lung homoge- nate presented inhibition （P〈0.01） in the drug group and the expression of MMP-9, MMP-2 mRNA was also depressed than that of the I/R group. CONCLUSION： Jidesheng Sheyao pian can inhibit the expression and activity of MMP-9 and MMP-2, attenuate basement membrane deg radation, sequentially, has protective effects on lung ischemia-reperfusion injury in rats.

关 键 词：季德胜蛇药片 肺缺血-再灌注损伤 基质金属蛋白酶 

分 类 号：R965.2[医药卫生—药理学]