顶空气相色谱法快速检测生活用纸中的大肠菌群  

Rapid Determination of Coliforms in Tissue Paper by Headspace Gas Chromatography

作　　者：田迎新[1] 胡会超[1] 陈春霞 柴欣生[1,2] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640 [2]国家纸制品质量监督检验中心,广东东莞523080

出　　处：《中国造纸》2013年第11期32-36,共5页China Pulp & Paper

基　　金：国家自然科学基金(21076091);国家质检总局科技计划(质量技术监督)项目(2011QK303)的资助

摘　　要：基于大肠菌群在培养过程中生长繁殖数量与其代谢产物CO2释放量的关系,建立了一种利用顶空气相色谱技术快速检测生活用纸中大肠菌群的新方法。该方法的初发酵检测需要14 h,复发酵检测需要4 h,即得到一个阳性结果只需要18 h,与传统的方法 (需要72 h)相比,大大提高了检测的效率。This paper reported a headspace gas chromatographic technique for rapid determination of coliforms in tissue paper. The method was based on the relationship between the number of coliforms and the mass of carbon dioxide （ CO2 ） produced during the bacteria incuba- tion. The results showed that the present method took only 18 hours for getting a positive result, The present method was simple, accurate and much efficient for coliforms testing compared with the traditional method.

关 键 词：顶空气相色谱 生活用纸 大肠菌群 快速检测 

分 类 号：TS77[轻工技术与工程—制浆造纸工程]