施用螯合剂对黄芥生长及土壤酶活性的影响  

Influence of Application of Chelating Agents on the Growth of Yellow Mustard and Enzyme Activity in the Soil

作　　者：祝方[1] 张飞[1] 杨晨[1] 陈雨[1] 

机构地区：[1]太原理工大学环境科学与工程学院,山西太原030024

出　　处：《土壤通报》2013年第6期1465-1469,共5页Chinese Journal of Soil Science

基　　金：山西省基础研究计划项目(2008011020);山西省教育厅科技开发项目(20090012)资助

摘　　要：通过盆栽实验,研究了螯合剂诱导植物修复重金属Pb污染土壤中Pb总量变化、黄芥生长及对土壤中过氧化氢酶、蔗糖酶、脲酶活性的影响。在重金属Pb污染土壤中施入草酸和EDTA,草酸促进了黄芥的生长,但EDTA降低了黄芥生物量。草酸和EDTA浓度分别为2 mmol kg-1和5 mmol kg-1,Pb浓度最低,处理效果最好。螯合剂诱导修复土壤,对过氧化氢酶活性有抑制作用,但加入EDTA,Pb含量与过氧化氢酶活性呈显著正相关;促进了蔗糖酶的活性,而Pb总量对酶活性为显著负影响,总量越大,蔗糖酶活性越小;不同浓度的螯合剂对土壤脲酶活性产生的效果不同。结果表明,草酸和EDTA对三种酶活性的抑制率顺序为过氧化氢酶>脲酶>蔗糖酶,EDTA对酶活性的促进效果优于草酸。在不抑制酶活性的基础上,蔗糖酶可作为评价草酸和EDTA修复重金属Pb污染土壤能力的重要指标。Through pot experiment, we investigated the variation of Pb concentration and the growth of yellow mustard as well as their influences on the activities of catalase, invertase and urease in the Pb contaminated soil with chelate-induced phytoremediation. The addition of oxalic acid promoted the growth of yellow mustard in Pb contaminated soils, while EDTA made yellow mustard biomass decrease. When the concentrations of oxalic acid and EDTA were separately 2 and 5 mmol kg-1, the concentration of Pb was lowest and optimum treatment effect was obtained. In the soil with chelate-induced remediation, the activity of catalase was inhibited. However, after EDTA addition, a significant positive correlation was observed between Pb content and the activity of hydrogen peroxide enzyme. The remediation promoted the activity of the sucrase, but the total amount of Pb on the activity of sucrase had a significant negative influence, greater amount of Pb led to lower sucrose activity. The effect of different concentrations of chelating agents on soil urease activity was different. Under the treatment of oxalic acid or EDTA, the order of the inhibition rate of three enzyme activities was catalase〉 urease〉 sucrase. On the basis of no inhibition of enzyme activity, the sucrase could be used as an important remediafion in heavy metal Pb contaminated soil. indicator of evaluating the ability of oxalic acid and EDTA remediation in heavy metal Pb contaminated soil.

关 键 词：草酸 EDTA 重金属PB 土壤 酶活性 

分 类 号：X131[环境科学与工程—环境科学]