三氧化二砷联合氟尿嘧啶对人肝癌SMMC-7721细胞Bcl-2和Bax表达的影响  

EFFECTS OF ARSENIC TRIOXIDE COMBINED WITH5-FU ON THE EXPRESSION OF BCL-2 AND BAX IN HUMAN LIVER CANCER CELL LINE SMMC-7721

作　　者：孙秉赋[1] 张博[2] 栾明翰[3] 

机构地区：[1]河北联合大学附属医院普通外科,河北省唐山市063000 [2]河北联合大学冀唐学院 [3]河北联合大学附属医院急诊科

出　　处：《中国煤炭工业医学杂志》2013年第11期1861-1863,共3页Chinese Journal of Coal Industry Medicine

基　　金：河北省科技支撑计划项目(编号:11276103D-33)

摘　　要：目的探讨三氧化二砷联合氟尿嘧啶(5-FU)对肝癌细胞凋亡相关因子Bcl-2和Bax表达的影响。方法体外培养人肝癌SMMC-7721细胞株,将细胞分为四组,即As2O3组、5-FU组、As2O3+5-FU组及对照组,采用免疫细胞化学法检测各组SMMC-7721细胞Bcl-2、Bax蛋白的表达。结果 As2O3组、5-FU组、As2O3+5-FU组细胞Bcl-2蛋白表达较对照组明显降低,Bax蛋白表达明显升高(P<0.01);联合用药组细胞Bcl-2蛋白表达较单用药组明显降低,Bax蛋白表达明显升高(P<0.01)。结论 As2O3联合5-FU可下调Bcl-2蛋白的表达,上调Bax蛋白的表达,从而诱导肝癌细胞凋亡。Objective To explore the effects of arsenic trioxide combined with 5 - FU on the expressions of apoptosis factors Bcl- 2 and Bax of liver cancer cell line. Methods Cultured human liver cancer cell line SMMC-7721 was divided into four groups in vitro： As203 group, 5- FU group, As203 plus 5- FU group and control group, the expressions of Bcl- 2 and Bax of these cells were examined by immunocytochemistry. Results The expression of Bcl - 2 was significantly lower in the As203 group, 5 - FU group and Asz 03 plus 5 - FU group than the control group, the expression of Bax was significantly increased（P〈 0.01）. The expression of Bcl - 2 was significantly lower in the combinational drug group than the single drug group, the expression of Bax was significantly increased （P〈 0. 01）. Conclusion Arsenic trioxide combined with 5 - FU can induce apoptosis of liver cancer cells by downregulating the expression of Bcl - 2 and upregulating the expression of Bax

关 键 词：三氧化二砷 5-氟尿嘧啶 肝癌 BCL-2 BAX 

分 类 号：R735.7[医药卫生—肿瘤]