刺芹侧耳与秀珍菇细胞融合及融合子的鉴定  

Fusion of Pleurotus eryngii and Pleurotus geesteranus Protoplasts and Identification of Fusant

作　　者：张鹏 龚玲凤 朱坚[2,3] 庄卫东 郑金水 林金 

机构地区：[1]泉州农业科学研究所,福建泉州362212 [2]福建农林大学菌物研究中心,福建福州350002 [3]福建农林大学园艺学院,福建福州350002

出　　处：《食用菌学报》2013年第3期1-5,共5页Acta Edulis Fungi

基　　金：福建省科技重大专项(编号:2008SZ0002)的部分研究内容

摘　　要：以刺芹侧耳(Pleurotus eryngii)双核菌株pl.e0091和秀珍菇(Pleurotus geesteranus)单孢菌株pl.g0001..1为亲本,分别制备原生质体,pl.e0091原生质体50℃水浴20min热灭活后与pl.g0001..1原生质体融合(25%PEG融合剂、pH=8、30℃水浴下融合20min),通过锁状联合和拮抗试验得到1株融合子,经RAPD、ISSR分子标记证明融合子含有双亲遗传物质。Protoplasts of a commercial strain of Pleurotus eryngii （pl. e0091） were inactivated by incubation at 50 C for 20 min, mixed with an equivalent concentration of protoplasts of a monokaryotic strain of Pleurotus geesteranus （pl. g0001.. 1）, and PEG added to the suspension mixture to a final concentration of 25% and pH 8.0. After incubating at 30 ~C for 20 min, the mixture was diluted and aliquots spread on to plates of regeneration medium. After subculturing isolated colonies on to potato dextrose agar and incubating at 25 C for five days, a protoplast fusant （R1） was selected on the basis of clamp connection formation and hyphal antagonism with the parent strain pl. e0091. RAPD and ISSR analyses confirmed that R1 generated DNA markers common to both parent stains.

关 键 词：刺芹侧耳 秀珍菇 原生质体融合 分子标记 

分 类 号：S646[农业科学—蔬菜学]