Screening of Prolific Micromonospora carbonacea in Antibiotics Production by Sodium Nitrite Mutagenesis  

抗生素高产炭样小单孢菌的亚硝酸盐诱变育种(英文)

作　　者：吴越 黄运红[1] 李瑾[1] 龙中儿[1] 

机构地区：[1]江西师范大学生命科学学院,江西南昌330022 [2]南昌市疾病预防控制中心,江西南昌330038

出　　处：《Agricultural Science & Technology》2013年第10期1409-1412,共4页农业科学与技术（英文版）

基　　金：Supported by Science and Technology Planning Project of Jiangxi Province of China(20112BBF60026)~~

摘　　要：[Objective] The purpose of the study is to breed Micromonospora car- bonacea highly producing antibiotics and then to improve the antibiotic production. [Method] Sodium Nitrite mutagenesis, combined with rifampicin resistance screening, was used in mutation breeding of M. carbonacea highly producing antibiotics from the strain of M. carbonacea JXNU-I. [Result] The overproducing strain JXNU-1-16- Y65 was screened with the production of antibiotics 266.05% more than that of the original strain. [Conclusion] The effectiveness of sodium nitrite mutation in breeding microorganisms highly producing antibiotic was proved, and the study may lay the foundation on further development and application of the antibiotic from M. car- bonacea JXNU-1.[目的]选育抗生素高产的炭样小单孢菌突变株,以提高炭样小单孢菌JXNU-1抗生素的产率。[方法]用亚硝酸钠对产抗生素的炭样小单孢菌JXNU-1单胞子悬浮液进行诱变处理,结合利福平抗性筛选法筛选。[结果]获得1株抗生素产率比出发菌株提高266.05%的抗生素高产突变株JXNU-1-16-Y65。[结论]研究证实了亚硝酸钠在诱变选育抗生素高产突变株中的应用价值,同时为炭样小单孢菌JXNU-1抗生素的开发应用奠定基础。

关 键 词：Micromonospora carbonacea Sodium nitrite mutagenesis Resistance screening 

分 类 号：TQ927[轻工技术与工程—发酵工程]