肺癌组织中HPV相关蛋白E6和E7与CD4＋和CD8＋T细胞相关性研究  

Relationship between human papillomavirus associated protein E6, E7 and CD4＋ and CD8＋ T cell in lung cancer

作　　者：成潇[1] 尹小文[1] 杜艳萍[1] 江兴堂[1] 

机构地区：[1]厦门大学附属中山医院呼吸内科,361004

出　　处：《国际呼吸杂志》2013年第22期1681-1685,共5页International Journal of Respiration

基　　金：厦门市科技计划社会发展项目（3502220114013）

摘　　要：目的探讨人乳头瘤病毒（HPV）相关蛋白E6和E7及CD4＋和CD8＋T细胞在肺癌组织中的表达及二者的关系。方法采用免疫组织化学法检测107例肺癌患者术后组织中HPV相关蛋白E6和E7及CD4＋和CD8＋T细胞的表达水平。结果107例肺癌组织中HPV相关蛋白的阳性表达率为57．94％（62／107），HPV相关蛋白表达与肺癌分化程度及吸烟史相关（x^2值分别为9．483和6．683，P值均〈O．05）。HPV阳性表达组与阴性表达组的CD4＋和CD8＋T细胞的表达均存在显著性差异（x^2值分别为26．533和21．266，P值均〈0．05），HPV相关蛋白表达与CD4＋和CD8＋T细胞的表达呈正相关（Rs=0．446，P〈0．05；Rs=0．407，P〈0．05）。结论HPV感染可引起肺癌患者机体特异性炎症反应，为肺癌的肿瘤免疫治疗提供理论基础。Objective To investigate the expressions of human papillomavirus （HPV） related protein E6,E7 and CD4＋ ,CD8＋ T cell in lung cancer and their relationship. Methods The expressions of HPV E6, E7 and CD4＋ ,CD8＋ T cell in 107 cases of lung cancer were detected by immunohistochemistry. Results The positive expression rate of HPV in lung cancer was 57.94% （62/107）. The expression of HPV was related with degree of differentiation of lung cancer and history of smoking （ x^2 = 9. 483, P G 0.05; x^2=6. 683, P G0.05）. The expression levels of CD4＋ T cell and CD8＋ T cell were significantly different between the positive HPV group and the negative HPV group （ 2：2 ： 26. 533, P 〈0.05; 2 ： 21. 266, P 〈0.05）. There was positive correlation between the expression of HPV and levels of CD4＋ T cell and CD8＋ T cell in lung cancer patients （ Rs=0. 446, P 〈0.05 ; Rs=0. 407, P 〈0.05）. Conclusions HPV infection may cause the specific inflammatory response in lung cancer patients. That may provide a theoretical basis for lung cancer immunotherapy.

关 键 词：肺癌 人乳头瘤病毒 CD4+T细胞 CD8+T细胞 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]