维吾尔族及汉族健康人群血清蛋白质谱的比较研究  

The study of serum protein difference between in Han nationality and Uygur nationality healthy persons

作　　者：张涛[1] 木拉提.热夏提 威力江.赛买提 王玉杰[2] 

机构地区：[1]新疆医科大学第二附属医院,830028 [2]新疆医科大学第一附属医院

出　　处：《山西医药杂志（下半月）》2013年第11期1211-1213,共3页Shanxi Medical Journal

基　　金：国家自然科学基金(81060210)

摘　　要：目的观察汉族与维吾尔族健康人群之间血清蛋白表达谱的变化。方法对30名维吾尔族及50名汉族健康体检者血清,采用弱阳离子交换蛋白芯片(CM10)和表面增强激光解吸离子化飞行时间质谱技术检测蛋白谱。结果汉族和维吾尔族间血清蛋白质谱存在明显差异,其中汉族组中质荷比为1 280、2 747的2个蛋白质丰度显著高于维吾尔族组(P<0.05)。结论维吾尔族与汉族健康人群之间的血清蛋白质指纹图谱有较明显差异,对建立新疆地区血清蛋白质指纹图谱疾病诊断模型具有参考价值。Objective To compare serum protein profiles in Han nationality and Uygur nationality healthy persons. Methods Serum samples from healthy persons （50 Han and 30 Uygur subjects） were detected by weak cation exchange （CM10） protein chip assay using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry（SELDI-TOF-MS） technology. Results Two peaks bearing mass to charge ratios of 1 280 and 2 747 were significantly higher in Han while lower in Uygur healthy persons （ P d0.05）. Conclusion Han and Uygur healthy persons in Xinjiang exhibited different fingerprints of serum proteins, such a difference will be significant to build a diagnostic model of diseases based on serum proteomics in Xinjiang.

关 键 词：汉族 维吾尔族 蛋白质组学 表面增强激光解吸电离飞行时间质谱 

分 类 号：R735.34[医药卫生—肿瘤]