热休克蛋白60对动脉粥样硬化大鼠心肌血管内皮生长因子和缺氧诱导因子-1α表达的影响  

Effect on the expression of VEGF and HIF-1 alpha in atherosclerosis rat myocardial with HSP60

作　　者：郝春艳[1] 段虎斌[1] 张军锋[2] 宋玲花[3] 

机构地区：[1]山西医科大学第一医院,030001 [2]山西医科大学公共卫生学院 [3]山西医科大学附属山西省心血管病医院

出　　处：《山西医药杂志（下半月）》2013年第11期1217-1219,共3页Shanxi Medical Journal

基　　金：山西省高校科技开发项目(2011004;2006115);山西省大学生创新基金(20103053);2013年山西医科大学博士启动基金(B0320 1217)

摘　　要：目的研究口服热休克蛋白60(HSP60)对动脉粥样硬化(AS)大鼠心肌血管内皮生长因子(VEGF)和缺氧诱导因子-1α(HIF-1α)表达的影响。方法 40只4周龄健康雄性SD大鼠,体质量(100±10)g。随机分为4组:健康对照组(C组),AS未干预组(AS组),造AS模型前口服HSP60组(H1组),造模后口服HSP60组(H2组),以上4组每组10只。本实验采用高脂饲料造AS模型,于大鼠180日龄时采血后处死,并取大鼠心肌作为标本。反转录-聚合酶链反应(RT-PCR)和免疫组织化学检测心肌VEGF和HIF-1α表达情况。结果C组、AS组、H1组、H2组大鼠心肌VEGF mRNA表达分别为:0.37±0.04,0.87±0.05,0.44±0.03,0.58±0.04;HIF-1αmRNA表达分别为:0.46±0.06,0.94±0.03,0.55±0.05,0.62±0.06;VEGF免疫组织化学阳性表达分别为:4.5±0.5,9.2±0.6,5.3±0.4,8.1±0.5;HIF-1α免疫组织化学阳性表达分别为:5.8±0.7,13.9±0.4,7.0±0.6,10.4±0.8。结论 AS大鼠心肌VEGF和HIF-1α表达较正常对照组增高,口服HSP60能降低VEGF和HIF-1α表达,即减轻心肌缺血缺氧性损伤和血管内皮炎症反应程度,而且越早口服,效果越好。Objective To study the effect on the expression of vascular endothelial growth factor （VEGF） and hypoxia inducing factor-1 alpha （HIF-1 alpha） in atherosclerosis（AS） rat myocardial with oral heat shock pro- tein 60 （HSP60）. Methods Forty four weeks male SD rats, weight （100＋ 10）g, were randomly divided into 4 groups： the healthy control group （C group） atherosclerosis group （AS group）, oral HSP60 group before building AS model （H1 group）, oral HSP60 group after building AS model （H2 group）, 10 rats in each group. AS model was made by high fat feed. At 180 days age, the rats were executed after obtaining the rats blood and myocardial. The gene and protein expression of rats myocardial VEGF and HIF-1 alpha were detected by reverse transcription- polymerase chain reaction （RT-PCR） and immunohistochemical. Results The VEGF gene expression in rats myo- cardial of C group, AS group, Hlgroup and H2 group respectively are： 0.37＋0.04, 0.87＋0.05, 0.44＋0.03 and 0. 58＋0. 04; the gene expression of HIF-1 alpha respectively are： 0. 46＋0. 06, 0. 94＋0. 03, 0.55＋0.05 and 0.62 ＋ 0.06; the protein expression of VEGF respectively are： 4.5 ＋ 0.5, 9.2＋ 0.6, 5.3＋0.4 and 8.1＋0.5 ; the protein expression of HIF-1 alpha respectively are： 5.8＋0.7,13.9＋0.4,7.0＋0.6 and 10.4＋0.8. Conclusion The gene and protein expression of VEGF and HIF -1 alpha in myocardial muscle of atherosclerotic rats enhanced compared with normal control group. Oral administration of HSP60 can reduce the gene and protein expression of VEGF and HIF-1 alpha in myocardial muscle , and reduce the degree of myocardial ischemia anoxic injury and vascular endothelial inflammatory response. Moreover, the earlier oral, the better results.

关 键 词：监控蛋白60 动脉硬化 血管内皮生长因子A 缺氧诱导因子-1Α 

分 类 号：R735.34[医药卫生—肿瘤]