去氢木香内酯特异性抑制Saccharomyces cerevisiae细胞壁中β-葡聚糖的合成  

Studies on the specific inhibition of dehydrocostus lactone againstβ-glucan synthesis in cell wall of Saccharomyces cerevisiae

作　　者：汪琨[1] 徐峥[1] 朱廷恒[1] 汪倩雯 崔志峰[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,硕士讲师浙江杭州310032 [2]浙江省食品药品检验研究院,浙江杭州310004

出　　处：《食品与发酵工业》2013年第10期29-34,共6页Food and Fermentation Industries

基　　金：浙江省自然科学基金资助项目(No.LQ13C140002)

摘　　要：基于酿酒酵母(Saccharomyces cerevisiae)及其细胞壁合成酶缺陷突变株组成的靶标定向全细胞筛选模型,对去氢木香内酯抗真菌的作用机制进行研究,发现去氢木香内酯特异性抑制β-葡聚糖合酶基因缺陷突变株Δfks1,其最低抑制浓度MIC值为16μg/mL,比野生型WHU2a和几丁质合酶基因缺陷突变株均低2倍;其对WHU2a生长的抑制能被山梨醇等渗溶液部分挽救;IC20浓度的去氢木香内酯处理后WHU2a的β-葡聚糖含量降低了16.83%,512μg/mL去氢木香内酯抑制了26.66%的β-葡聚糖合酶活性。研究结果表明,去氢木香内酯能特异性抑制真菌细胞壁中葡聚糖的合成,从而抑制真菌细胞生长。The antifungal mechanism of dehydrocostus lactone （DHCL） was studied using the target-directed whole cell screen pattern consisting of wild-type Saccharomyces cerevisiae strain WHU2a and three cell wall synthasedeficient strains Afksl, CHS020 and CHS003 derived from WHU2a. The results showed that DHCL could specifically inhibit the growth of glucan synthase-deficient strain Afksl , and the minimum inhibitory concentration （MIC） was 16 μg/mL, which was 2 folds lower than that against the wild-type strain and chitin synthase-deficient strains. The growth inhibition on WHU2a could partly be rescued by sorbitol through the growth rescue assay. Furthermore, the content of β-glucan on WHU2a cell wall was decreased by 16.83% after treated with IC20 of DHCL, the activity of β- glucan synthase was inhibited by 26.66% after treated with 512 μg/mL of DHCL. Based on the data presented, it is reasonable to conclude that the specific inhibition on the β-glucan synthesis in cell wall is one of the main antifungal mechanisms of DHCL.

关 键 词：去氢木香内酯 抗真菌作用 葡聚糖合酶 SACCHAROMYCESCEREVISIAE 

分 类 号：TS201.2[轻工技术与工程—食品科学]