鼠李糖乳杆菌KF7产单胺氧化酶抑制剂培养条件的优化  

Optimization of the cultural conditions for Lactobacillus rhamnosus KF7 to biosynthesize mono-amine oxidase inhibitors by response surface methodology

作　　者：孟春 吴正钧[1] 郭本恒[1,2] 韩瑨[1] 

机构地区：[1]光明乳业股份有限公司乳业研究院乳业生物技术国家重点实验室,上海200436 [2]上海海洋大学食品学院,硕士研究生上海201306

出　　处：《食品与发酵工业》2013年第10期107-112,共6页Food and Fermentation Industries

基　　金：国家高技术研究发展计划(863计划)(2011AA100901);国家"十二五"科技支撑计划(2013BAD18B01)

摘　　要：单胺氧化酶(MAO)是体内具有代谢作用的一种黄素蛋白酶,单胺氧化酶在体内活性或含量的异常增高会导致精神和神经异常疾病,因而作为治疗此类疾病的MAO抑制剂(MAOIs)受到广泛关注。鼠李糖乳杆菌KF7在脱脂乳中发酵后的发酵液具有MAO抑制作用,为得到具有较高MAO抑制作用的发酵液,采用Box-Behnken响应面实验设计对鼠李糖乳杆菌KF7在脱脂乳中的发酵条件进行优化,并且选取接种量、培养温度、培养时间进行3因素3水平实验设计。通过结果分析得到较优的发酵条件:接种量3.29%,培养温度34.71℃,培养时间74.4 h,MAO抑制率理论值72.18%,验证值为71.98%。Monoamine oxidases （MAOs）, which are flavoprotein enzymes, are responsible for catabolism of monoamine substance in vivo. The hyperactivity and high level of monoamine oxidases will lead to some mental illness and neurodegenerative diseases. Monoamine oxidase inhibitors, as the drug for neurodegenerative diseases, were attracted much attention. Skim milk fermented by LactobaciUus rhamnosus KF7 possessed strong inhibitory activity to monoamine oxidase in vitro. To facilitate the purification of active components, the cultural conditions for Lactobacillus rhamnosus KF7 to biosynthesize monoamine oxidase inhibitors in skim milk were optimized by response surface analysis （RSA）. The optimum conditions for Lactobacillus rhamnosus KF7 to biosynthesize MAOIs in skim milk was obtained as follows： inoculum concentration 3.29% , culture temperature 34.71℃, culture time 74.4h. Under the optimized conditions, the estimated value and verified values for inhibitory rate were 72.18% and 71.98% , respectively.

关 键 词：鼠李糖乳杆菌KF7 单胺氧化酶(MAO) 抑制率 响应面法 

分 类 号：TQ925[轻工技术与工程—发酵工程]