硒化壳聚糖增强K562/ADM细胞对ST1571敏感性的作用研究  

Effect of Selenium Chiston on Enhancing Chemotherapy Sensitivity of ST1571 to Human Leukemia multidrug resistance K562 /ADM Cell Line

作　　者：邓守恒[1] 王贤和[1] 李芳[1] 曹风军[1] 蔡晓军[1] 陈萍[1] 

机构地区：[1]湖北医药学院附属人民医院肿瘤中心,湖北十堰442000

出　　处：《时珍国医国药》2013年第11期2610-2612,共3页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省自然科学基金(No.2008CDZ048);湖北医药学院基金(No.2008QDJ1)

摘　　要：目的研究硒化壳聚糖增强ST1571对多药耐药白血病K562/ADM细胞敏感性的作用,并探讨其逆转耐药的可能机制。方法硒化壳聚糖作用K562/ADM细胞,MTT法检测ST1571对K562/ADM细胞增殖的影响;RT-PCR法和免疫印迹法检测mdr-1/P-gp表达的改变。结果单独应用1μmol·L-1ST1571作用124 h,对K562/ADM的增殖抑制率为(24.35±2.37)%,加入100 mg·L-1或200 mg·L-1硒化壳聚糖后,抑制率则上升为(62.79±4.89)和%(78.63±5.42)%,逆转倍数分别为2.51倍和3.43倍;ST1571与100 mg·L-1或200 mg·L-1硒化壳聚糖联用可使mdr-1mRNA水平分别下调(60.93±5.21)%和(82.61±6.74)%(P<0.01),使P-gp表达水平分别下调(58.35±5.38)%和(81.14±8.96)%(P<0.01)。结论硒化壳聚糖能够增强K562/ADM细胞对ST1571的敏感性,下调其mdr-1mRNA和P-gp表达水平,从而起到逆转K562/ADM白血病细胞对ST1571的多药耐药作用。Objective To Study the effect of Selenium chiston （Sc） on enhancing chemotherapy sensitivity of ST1571 to I（562/ ADM ceils and to reveal its mechanism . Methods MrIT method was used to observe the alteration of the proliferation of K562/ ADM cell line treated with ST1571 alone or combined with Sc. The expression level of P - gp was determined by Western Blot and the transcription of mdr -1 gene was detected by semi - quantitative RT - PCR after ST1571 alone or combined with Sc treatment. Results The cytotoxie effect of ST1571 combined with Sc on K562/ADM cell line was significantly higher than that of ST1571 alone （P 〈 0.01 ）. After treated with 1μmol·L-1 ST1571 combined with 100 or 200 mg·L-1 of Sc ,the synergistic interaction on K562/ADM cell line increased 2.51 and 3.43 times;the expression of mdr - 1 gene was significantly reduced by （60.93 ± 5. 21 ） % and （82.61 ± 6.74 ） % （P 〈 0.01 ） ; and P - gp expression was deregulated by （58.35 ± 5.38 ） % and （ 81.14 ± 8.96 ） % （P 〈0.01 ） . Conclusion Sc could obviously enhance the antineoplastic effect of ST1571 on K562/ADM cells by reducing mdr^-1 genetransription and blocking the P - gp protein expression.

关 键 词：硒化壳聚糖 K562 ADM细胞 ST1571 多药耐药 

分 类 号：R733.7[医药卫生—肿瘤]