蒙药用植物耧斗菜的体内抗氧化活性研究  

Study on Antioxidant Activity of Aquilegia viridiflora.

作　　者：敖恩宝力格[1] 张园园[1] 乌达巴拉[1] 

机构地区：[1]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022

出　　处：《时珍国医国药》2013年第11期2644-2645,共2页Lishizhen Medicine and Materia Medica Research

基　　金：内蒙古自治区高等学校科研项目(No.NJ10043);内蒙古自治区自然科学基金项目(No.2010BS0504);教育部科学技术发展重点项目(No.210042)

摘　　要：目的研究蒙药用植物耧斗菜的体内抗氧化作用。方法采用80%乙醇对耧斗菜进行超声波提取。检测提取物对黑腹果蝇寿命及其过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的影响。结果各浓度组耧斗菜提取物使雌雄果蝇SOD活性上升,且浓度越大,促进作用越强(P<0.05);雄蝇MDA含量降低,CAT活性升高(P<0.01或P<0.05),而对雌蝇MDA含量和CAT活性无显著影响。给药组雌雄果蝇与对照组比较可延长平均寿命(P<0.01或P<0.05),并能提高平均最高寿命。结论耧斗菜乙醇提取物具有较强的体内抗氧化活性,能延长果蝇寿命,增强黑腹果蝇体内抗氧化酶的活性并减少脂质过氧化产物MDA的生成。Objective The oxidation resistant components of Aquilegia viridiflora were extracted with 80% ethanol with ultra sonic wave. Methods Observe the effect of extract on the antioxidation and the life span in Drosophila melanogaster. Results SOD activities of male/female flies and CAT activities of male flies were increased and MDA concentrations of male flies were decreased significantly in Aquilegia viridiflora groups than those in control group （P 〈 0.05 or P 〈 0.01 ）. The average life span and average maximum life span of flies in 0.06 mg/100 g Aquilegia viridiflora group were increased significantly than those in control group （ P 〈 0.05 or P 〈 0.01 ）. Conclusion Conclusions Aquilegia viridiflora could increase antioxidative effect, reduce lipid peroxidation and prolong the life span in drosophila.

关 键 词：耧斗菜 抗氧化活性 蒙药用植物 

分 类 号：R29[医药卫生—民族医学]