GH基因遗传多态性与中国荷斯坦牛泌乳性状的遗传效应分析  

Associations of GH gene polymorphisms with milk production traits in Chinese Holstein

作　　者：马彦男[1,2] 贺鹏迦[1] 朱静[1] 雷赵民[2] 刘哲[2] 吴建平[2] 

机构地区：[1]甘肃民族师范学院化学与生命科学系,甘肃合作747000 [2]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《中国兽医学报》2013年第12期1943-1948,共6页Chinese Journal of Veterinary Science

基　　金：科技部星火计划资助项目(2008GA860014);甘肃省生物技术专项(GNSW-2009-08)

摘　　要：为分析GH基因SNPs与中国荷斯坦牛泌乳性状的相关性。以232头中国荷斯坦牛为研究材料,采用PCRSSCP技术检测GH基因第5外显子及其相邻区域的多态性,并进行相关性分析。结果发现GH基因第4内含子(2 017bp处)存在C→T的碱基转换,该群体在此位点处于Hardy-Weinberg平衡状态,为中度多态。与泌乳性状的相关性分析表明,该多态位点与泌乳性状显著相关(P<0.05),TT型305d产奶量、305d乳脂量、305d乳蛋白量和305d乳糖量显著高于CC型(P<0.05);TC型与TT型、CC型之间305d产奶量、305d乳脂量、305d乳蛋白量差异均不显著(P>0.05),但TC型在数值上有优于CC型的趋势。建议将等位基因T作为提高奶牛产奶量、乳脂量和乳蛋白量的候选分子标记。Two hundred and thirty two Chinese Holstein cows were selected to study the correlation of GH gene SNPs and milk production traits. Using PCR-SSCP method to detect the polymor- phisms of exon5 and adjacent domains of GH gene,than made the study of the correlation analy- sis. The results showed that there exist C-~T mutation in intron4,it is moderate polymorphic and Hardy-Weinberg equilibrium. The correlation analysis of milk production traits showed that this SNPs has significant correlation with milk production traits,305 d milk yield,305 d milk fat yield, 305 d milk protein yield and 305 d lactose of TT genotype were significantly higher than thoes of CC genotype;there is no significant difference in 305 d milk fat yield, 305 d milk protein yield of TC,CC and TT type,but TC type has a higher numeric trend than CC type. T allele can be consid- ered as a genetic marker to improve milk yield,milk fat yield,and milk protein yield.

关 键 词：奶牛 生长激素(GH)基因 单核苷酸多态性 泌乳性能 

分 类 号：S852.2[农业科学—基础兽医学]