芽孢杆菌H-1菌株的固定化及降解动力学研究  

Immobilization and Degradation Kinetics of Bacillus H-1 Strain

作　　者：辛蕴甜[1] 赵晓祥[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《环境科学与技术》2013年第11期67-73,共7页Environmental Science & Technology

基　　金：上海市基础研究重点项目(09JC1400600)

摘　　要：采用海藻酸钠-海藻酸钙法对芽孢杆菌H-1菌株进行包埋实验,测试各固定化因素对凝胶微球物理性能的影响,得到各因素合适的取值范围。以石油降解率为试验指标对固定化因素进行4因素3水平正交试验,结果显示菌株的最佳固定化条件为海藻酸钠浓度7%、菌悬液添加量75%、CaCl2浓度2%、交联时间24 h。对比固定化菌株与游离菌株的降解效果,结果显示固定化菌株的降解效率大于游离菌株。对固定化菌株的降解动力学进行研究,结果表明当石油浓度较低时,固定化菌株的降解动力学方程符合Monod方程的线性简化形式,方程的半饱和常数K S=52.33 mg/L,最大反应速率v max=44.05 d-1。当原油浓度较高时,其降解速率大于方程的v max。In this paper immobilization of Bacillus H-1 referred to the use of SA（sodium alginate）and CA（calcium alginate）method and the tests of immobilization factors that affected performance of gel microspheres in order to find a suitable range of the each factor. Degradation rate of petroleum oil was used as an experimental index and a four-factor orthogonal test was conducted at three levels;thereafter the optimal condition for immobilization was worked out. Compared with free strain of Bacillus,the immobilized strain was better with respect to degradation efficiency. As to the degradation kinetics of the immobilized strain,the result of the studies indicated that when oil concentration was relatively low the kinetic model could be expressed in the simplified linear form of Monod equation with the half-saturation constant 52.33 mg/L,and the maximum reaction rate 44.05 d-1.

关 键 词：芽孢杆菌H-1菌株 固定化 凝胶微球 正交试验 降解动力学 MONOD方程 

分 类 号：X172[环境科学与工程—环境科学]