新型褪黑素受体激动剂Neu-p11对缺血/再灌注损伤大鼠心肌细胞凋亡的影响  

Protective effects of Neu-p11 on myocardial ischemia-reperfusion injury in rats

作　　者：黄靓[1,2] 张弛[1] 王汉群[2] 向琼[2] 佘美华[3] 何平平[1] 尹卫东[1] 

机构地区：[1]南华大学心血管疾病研究所,湖南衡阳421001 [2]南华大学外总教研室,湖南衡阳421001 [3]南华大学生物化学与分子生物学教研室,湖南衡阳421001

出　　处：《中国药理学通报》2013年第12期1660-1663,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81200590);湖南省教育厅资助科研项目(No 11C1093;11C1095)

摘　　要：目的研究Neu-p11预处理对大鼠心肌缺血/再灌注损伤细胞凋亡的影响。方法 30只♂SD大鼠随机分为3组:假手术组,缺血/再灌注损伤组和Neu-p11预处理组。测定血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)的活性及心肌丙二醛(MDA)的含量,TUNEL法检测心肌细胞凋亡指数,免疫印迹法检测Bcl-2、Bax蛋白的表达。结果 Neu-p11预处理可明显改善缺血/再灌注损伤大鼠的心功能损害,减少血清中LDH、CK的释放和心肌组织中MDA的产生,可使心肌细胞凋亡率减少,心肌Bcl-2蛋白表达上调,Bax蛋白表达下调。结论 Neu-p11对心肌缺血/再灌注损伤具有保护作用,其机制可能与抑制细胞凋亡有关。Aim To study the effect of Neu-p11 on myocardial ischemia-reperfusion injury in rats. Meth- ods Thirty male Wistar rats were randomly divided into three groups： sham group,I/R group and Neu-p11 group. LDH and CK activity in serum and MDA con- tent in myocardial tissue were measured. Cell apoptosis was evaluated by TUNEL assay. Bcl-2 and Bax protein expressions were detected by Western blot. Results Neu-pl 1 significantly decreased the level of LDH, CK and MDA content. The myocardial apoptotic index in Neu-pll group was markedly lower than that in I/Rgroup. And Western blot analysis revealed that the ex- pression of Bcl-2 significantly increased, while Bax ex- pression significantly decreased in Neu-pll group, compared with those in I/R group. Conclusion Neu- pll shows protective effects against myocardial ische- mia-reperfusion injury in rats, and the mechanism is probably related to its anti-apoptotic activity.

关 键 词：Neu—p11 缺血 再灌注 心肌细胞 细胞凋亡 大鼠 褪黑素受体激动剂 

分 类 号：R-332[医药卫生] R322.11