中枢阿片受体在远端缺血预处理对在体大鼠缺血后心脏保护中的作用  

Role of opioid receptors in the protective effects of remote ischemic preconditioning against myocardial ischemia-reperfusion injury in rats

作　　者：陆姚[1,2] 胡军[1] 张野[1] 董春山 余骏马 

机构地区：[1]安徽医科大学第二附属医院麻醉科,安徽合肥230601 [2]安徽医科大学第三附属医院麻醉科,安徽合肥230061

出　　处：《中国药理学通报》2013年第12期1707-1710,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金课题(No 81100105)

摘　　要：目的探讨脊髓中枢阿片受体在远端缺血预处理减轻大鼠心肌缺血/再灌注损伤中的作用。方法建立鞘内置管和心肌缺血/再灌注损伤模型。大鼠随机分为10组,每组6只:对照组(CON组);远端缺血预处理组(RIPC组);NM组(naloxone methiodide,非特异性阿片受体阻断剂,20μg·kg-1)、nor-BNI组(nor-binaltorphimine,κ阿片受体阻断剂,15nmol)、CTOP组(μ阿片受体阻断剂,15 nmol)和NTD组(naltrindole,δ阿片受体阻断剂,15 nmol);NM+RIPC组、nor-BNI+RIPC组、CTOP+RIPC组和NTD+RIPC组。远端缺血预处理是心脏缺血前经3个循环的左侧股动脉缺血5min和再灌注5 min。左冠状动脉缺血30 min,再灌注120min诱导心肌缺血/再灌注损伤。观察指标包括:血流动力学指标;心肌缺血危险区(AAR)、梗死区(IS)的体积、心肌梗死面积以IS/AAR表示。结果与CON组相比,RIPC组、nor-BNI+RIPC组和NTD+RIPC组的IS和IS/AAR均明显下降(P<0.01);与RIPC组比较,NM+RIPC组和CTOP+RIPC组的IS和IS/AAR均明显升高(P<0.01)。各组在各时点的MAP、HR和RPP比较差异无统计学意义(P>0.05)。结论脊髓中枢μ阿片受体可能参与介导远端缺血预处理减轻大鼠心肌缺血/再灌注损伤中的作用,并不涉及κ和δ阿片受体。Aim To investigate the role of spinal cord opioid receptors in the protective effects of remote is- chemic preconditioning against myocardial ischemia- reperfusion injury in rats. Methods Intrathecal cath- eter placement and myocardial ischemia-reperfusion models were established, and rats were randomly as- signed to 10 groups： control group（ CON ） , remote is- chemic preconditioning group （ RIPC ）; intrathecal naloxone methiodide group（ NM, a non-selective opi- oid receptor antagonist, 20 μg· kg^-1） , intrathecal nor-Binahorphimine group（ nor-BNI, a κ opioid recep- tor antagonist, 15 nmol）, intrathecal CTOP group （ CTOP, a μ opioid receptor antagonist, 15 nmol） , in- trathecal nahrindole group （ NTD, a δ opioid receptor antagonist, 15 nmol）, intrathecal 20 μg · kg^-1 NM ＋ RIPC group（ NM ＋ RIPC ） , intrathecal 15 nmol nor- BNI ＋ RIPC group （ nor-BNI ＋ RIPC）, intrathecal 15 nmol CTOP ＋ RIPC group （ CTOP ＋ RIPC ）, and intrath-ecal 15 nmol NTD ＋ RIPC group（ NTD ＋ RIPC ）. In- dicators to be observed were composed of hemodynamic values, the volume of area at risk（AAR） , infarct size （IS） , and the area of myocardial infarction, demon- strated by 1S/AAR. Results Compared with CON group, the volume of IS and IS/AAR was reduced in RIPC, nor-BNI ＋ RIPC and NTD ＋ RIPC group （P 〈 0.01 ） ; the protective effects of RIPC was abolished by pretreatment with NM and CTOP （P 〈 0.01 ）. There was no significant statistical difference in other observed data among all groups. Conclusion opioid receptor of spinal cord is probablely involved in the protective effects of remote ischemic preconditioning a- gainst myocardial ischemia-reperfusion injury.

关 键 词：阿片受体 脊髓 缺血 再灌注 远端预处理 心肌 股动脉 

分 类 号：R-332[医药卫生] R322.11