运动对高脂饮食大鼠下丘脑SOCS-3和BDNF以及瘦素抵抗的影响  

Effect of Swimming Exercise on Hypothalamus SOCS -3, BDNF and Leptin -resistance in Rat with High -fat Diet

作　　者：田雷[1] 陈钢[2] 

机构地区：[1]长春师范学院体育学院,吉林长春130032 [2]东北师范大学体育学院,吉林长春130024

出　　处：《广州体育学院学报》2013年第6期85-89,共5页Journal of Guangzhou Sport University

摘　　要：目的：观察运动对高脂饮食大鼠血清和下丘脑瘦素（Leptin）以及下丘脑瘦素长型受体（OB—Rh）、促黑皮素受体4（MC4R）、细胞因子信号转导抑制因子-3（SOCS-3）mRNA表达和脑源性神经营养因子（BDNF）的影响，探讨适宜运动对高脂饮食大鼠预防肥胖的部分中枢机制。方法：雄性sD鼠24只，随机分为正常对照组（C组），高脂模型组（H组）和高脂60min运动组（HE组），每组8只。高脂模型组和高脂60rain运动组大鼠喂饲高脂饲料，高脂饮食运动组大鼠进行60min的无负重游泳运动。持续10周。结果：10周运动后，高脂模型组大鼠较正常对照组大鼠体重、Lees指数、体脂、脂体比均显著增高（P〈0．05），血清瘦素水平显著升高（P〈0．05），下丘脑内OB—Rb、MCARmRNA表达量显著降低（P〈0．05），SOCS-3mRNA表达量显著升高（P〈0．05），下丘脑BDNF含量显著降低（P〈0．05）；高脂60rain运动组较高脂模型组大鼠体重显著降低（P〈0．01），LeeS指数、体脂、脂体比均显著降低（P〈0．05），血清瘦素水平显著升高（P〈0．05），下丘脑OB—Rb、MC4R、leptinmRNA表达量显著升高（P〈0．05），下丘脑SOCS-3mRNA表达量显著降低（P〈0．05），下丘脑BDNF含量显著升高（P〈0．05）。结论：长期适宜的运动可通过降低下丘脑SOCS-3的表达，增加下丘脑Leptin与受体OB—Rb的结合，降低了瘦素抵抗，激活下丘脑含MC4R受体的神经元，从而刺激BDNF的释放，从而起到预防高脂饮食引起肥胖的作用。Objective ： To explore the possible mechanism of exercises in high - fat diet induced rats with obesity, we observed the serum leptin, the mRNA expression of leptin, OB - Rb, MC4R and SOCS - 3 in hypothalamus, and the content of BDNF in hypothalamus. Method： 24 male Spragne -Dawley rats were randomly assighned to 3groups （each n = 8 ） ： control group （C Group）, high - fat model group （HE） and high - fat 60 - min exercise training group （ HE Group）, respectively. Rats of HE Group swam for 60 min each day without loading. Results ： After 10 weeks, to compared with the C group, the body weigh, Leeg index and the body fat of H group were obviously increased （P 〈 0. 05 ） , the serum leptin was significantly increased （P 〈 0.05 ） ; In hypothalamus, the mRNA expression of OB - Rb and MCAR were dramatically decreased （ P 〈 0.05 ） and the mRNA expression of SOCS - 3 was markedly increased （ P 〈 0. 05 ） , the concentration of BDNF was obviously decreased （ P 〈0.05 ）. To compared with the H group, the body weight, Lee~ index and the body fat of HE group was obviously lower （ P 〈 0.01 ） ; the serum leptin was significantly decreased （ P 〈 0.05） ; In hypothalamus, the mRNA expression of OB - Rb, leptin, and MCAR were dramatically increased （ P 〈 0.05 ）, and the mRNA expression of SOCS - 3 markedly decreased （ P 〈 0.05 ）, the concentration of BDNF was obviously increased （ P 〈 0.05 ）. Conclusion ： The appropriate long - term exercise can amelio- rate the ＂leptin resistance＂, the possible mechanisms is ： a long - term exercise can decrease the expression mRNA of SOCS - 3, then induced OB -Rb binding to leptin, with its activation to the hypothalamic neurons MC4R that causing a increase in BDNF production, thereby reducing food intake and stimulating the motor neuron to increase exercise training, so that in- creased energy expenditure.

关 键 词：运动 肥胖 细胞因子信号转导抑制因子-3 脑源性神经营养因子 瘦素抵抗 

分 类 号：G804.2[文化科学—运动人体科学]