血必净注射液对急性肺栓塞兔B型脑钠肽水平的影响  

The effect of Xuebijing injection on B-type natriuretic peptide level of rabbits with acute pulmonary thromboembolism

作　　者：陈立[1] 李兰[1] 吕波[3] 韩悦[2] 黄瑞峰[1] 

机构地区：[1]贵州省中医院,贵阳中医学院第一附属医院重症医学科,贵州贵阳550001 [2]贵州省中医院,贵阳中医学院第一附属医院产科,贵州贵阳550001 [3]贵阳中医学院,贵州贵阳550002

出　　处：《中国中西医结合急救杂志》2013年第6期345-348,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：基金项目：贵州省科技计划项目（黔科合J字[2010]2193号）

摘　　要：目的：探讨血必净注射液对急性肺栓塞（APE）的治疗作用及可能机制。方法选用新西兰大耳白兔24只，按随机数字表法分为对照组、APE模型组和血必净组，每组8只。采用自体血栓回输法建立APE模型；血必净组模型成功建立后于兔耳缘静脉注射血必净注射液1 mL/kg，每日2次，连续7 d；APE模型组和对照组给予等量生理盐水。采用酶联免疫吸附试验（ELISA）分别测定制模后8 h和1、3、5、7 d各时间点血浆B型脑钠肽（BNP）含量变化；7 d后取肺脏进行肺组织病理学观察。结果与对照组比较，制模后8 h APE模型组和血必净组BNP水平明显增高，以APE模型组最明显，差异均有统计学意义（均P＜0.05）；3 d时， APE模型组和血必净组BNP水平达峰值后逐渐下降，但APE模型组明显高于血必净组（ng/L：581.1±144.6比251.5±22.5），差异有统计学意义（P＜0.05）；7 d时血必净组BNP下降至接近于对照组水平（ng/L：78.5±9.4比50.9±11.5），APE模型组亦有所下降，但仍明显高于血必净组（ng/L：214.1±47.6比78.5±9.4），差异有统计学意义（P＜0.05）。肺组织病理学观察显示，血必净组梗死面积明显减小，有轻度的炎性细胞浸润；而APE模型组肺组织大部分坏死，其内见较多炎性细胞浸润。结论血必净注射液能够显著降低肺栓塞兔血浆BNP水平，同时减轻肺栓塞兔肺组织炎症浸润程度，减少梗死面积。Objective To explore the therapeutic effect and possible mechanisms of Xuebijing injection for treatment of acute pulmonary thromboembolism（APE）in rabbits. Methods Twenty-four New Zealand rabbits with big ears were randomly divided into control group,model group and Xuebijing group（each n=8）. The model of APE was performed by re-infusing of autologous blood clots. After the model was established,in the rabbits of the Xuebijing group,Xuebijing injection 1 mL/kg was injected into the ear marginal vein,twice daily for consecutive 7 days. In the model and control groups,the same amount of normal saline was given via the same route. The changes in plasma levels of B-type natriuretic peptide（BNP）were detected by enzyme-linked immunosorbent assay（ELISA） at the time points of 8 hours and 1,3,5,7 days,and after 7 days the lungs of the rabbits were removed for the lung histopathological examination. Results The BNP levels of the model group and Xuebijing group were significantly higher compared with the level of control group at 8 hours after embolization,the model group being the most significant（all P〈0.05）. On day 3,the BNP levels of the model group and Xuebijing group reached their peaks,and then gradually decreased,but the level of model group was significantly higher than that of Xuebijing group（ng/L：581.1±144.6 vs. 251.5±22.5,P〈0.05）. On day 7,the BNP level of Xuebijing group fell to the level of the control group（ng/L：78.5±9.4 vs. 50.9±11.5）,the level of the model group also decreased,but it was still much higher than that of the Xuebijing group（ng/L：214.1±47.6 vs. 78.5±9.4,P〈0.05）. Histopathological examination showed Xuebijing could significantly reduce the infarct size with mild inflammatory cell infiltration,but the infarct size of the model group was obviously bigger than that of the Xuebijing group and accompanied by relatively more inflammatory cell infiltration. Conclusion Xuebijing injection can significantly reduce the plasma BNP level of the rabbits with

关 键 词：血必净注射液 B型脑钠肽 兔 肺栓塞 肺组织病理 

分 类 号：R[医药卫生]