催化动力学光度法测定奶制品中微量三聚氰胺  

Catalytic Kinetic Photometric Determination of Trace Amount of Melamine in Dairy Products

作　　者：郭振良[1] 唐清华[1] 蒙延峰[1] 任淑华[1] 

机构地区：[1]鲁东大学化学与材料科学学院,烟台264025

出　　处：《理化检验（化学分册）》2013年第11期1349-1351,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：鲁东大学基金资助项目(042911)

摘　　要：在磷酸-磷酸二氢钾缓冲溶液中，痕量三聚氰胺对铁氰化钾氧化4-氨基安替比林的显色反应有催化作用，在显色产物的吸收峰510nm处测量时，所测得吸光度的增量△A与三聚氰胺的质量浓度在0．4～2．4mg·L-1叫范围内呈线性关系。据此提出了催化动力学光度法测定痕量三聚氰胺的方法。优化的显色反应条件如下：①20g·L-14-氨基安替比林溶液用量为2．5mL；②反应温度为70℃；③反应时间为4min。测得方法的检出限（3s）为0．313mg·L-1。以纯鲜牛奶为基体用标准加入法进行回收试验，方法的回收率在98．6％～101％之间，测定值的相对标准偏差（n=6）在0．13％～0．69％之间。It was found that in H3 PO4-KH2PO4 buffer solution, the oxidative coloration of 4-amino antipyrine by potassium ferricyanide was catalyzed by the presence of trace amount of melamine, and that linear relationship was kept between the magnitude of increase in absorbance （△A） and mass concentration of melamine in the range of 0.4-2. 4 mg . L -1 as measured at 510 nm, the absorption maximum of the colored product. Based on these facts, a catalytic kinetic photometric method for determination of trace amount of melamine in dairy products was proposed. The optimized conditions found for the color reaction were as follows：① amount of 20 g . L-1 4-amino antipyrine solution used： 2.5 mL; ② temperature of reaction： 70℃ ; ③time of reaction： 4 min. Detection limit （3s） found was 0. 313 mg . L -1. Using pure fresh milk as matrix, test for recovery was made by standard addition method, giving values of recovery in the range of 98. 6%- 101% and RSD＇s （n= 6） in the range of 0. 13%- 0. 69%.

关 键 词：三聚氰胺 催化动力学光度法 奶制品 

分 类 号：O643.32[理学—物理化学]