急性T淋巴细胞白血病Jurkat细胞株新转录本630序列的鉴定及其功能  

Identification and function analysis of a novel transcript 630 in acute T-cell lymphoblastic leukemia Jurkat cell lines

作　　者：殷丽萍[1] 张琳[1] 陆超[1] 

机构地区：[1]南京医科大学第一附属医院儿科,210029

出　　处：《中华实用儿科临床杂志》2013年第21期1643-1646,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金（81170487）

摘　　要：目的急性T淋巴细胞白血病（T—ALL）细胞株Jurkat新转录本630序列的鉴定及功能研究。方法提取Jurkat细胞RNA进行全转录组测序；应用反转录聚合酶链反应（RT—PCR）联合测序分析，对新转录630序列进行鉴定；RT—PCR检测630序列在不同组织中的表达谱；小干扰RNA（siRNA）转染Jurkat细胞，实时荧光定量PCR（Real．timePCR）检测630序列的转录水平；采用流式细胞仪测定630序列下调后对细胞凋亡的影响。CCK-8法检测细胞增殖。结果证实新转录本630序列存在；630序列是肿瘤相关性转录本，在JurkatE6—1、Hb60和肝癌等细胞中高表达，而在正常组织和细胞中不表达；降低T—ALL细胞中630序列转录水平后凋亡细胞比例变化不大，但细胞增殖受到抑制。结论630序列为T—ALL细胞株Jurkat细胞的新转录本，降低其转录水平能抑制细胞的增殖可能参与T—ALL的发生发展。Objective To identify and analyze a novel transcript 630 in acute T-cell lymphoblastic leukemia cell （T-ALL） Jurkat cell lines. Methods RNA-sequencing was applied to transcriptome profiling. The novel transcript 630 was identified by reverse transcription polymerase chain reaction （RT-PCR） combined with sequencing. The ex- pression level of the novel transcript was detected in some tumor cell lines and normal cells. The sequences for small in- terfering RNA （siRNA） against 630 were designed and synthesized. Then, the effects of siRNA were determined by real-time PCR. Apoptotic cells and cell cycle analysis were investigated with the flow cytometry ＂after siRNA-mediated knockdown of 630. Results The novel transcript 630 was identified. High expression level of 630 was detected in Jur- kat E6-1, HL60 and human umbilical vein endothelial（HUVE） cells. The knockdown of transcript 630 induced a de- crease of cellular proliferation. Conclusions The novel transcript 630 is one of the novel transcripts in Jurkat eells. The decreased expression level of transcript 630 can suppress the proliferation of leukemia cells. The novel transcript 630 might contribute to the treatment of T-ALL.

关 键 词：白血病 JURKAT细胞 转录组 

分 类 号：R733.7[医药卫生—肿瘤]