鼠脑反复缺血再灌注海马6种氨基酸、单胺递质及其代谢产物变化的研究  

A study of effect of repeated global ischemia by in vivo dialysis on extracellular concentrations of neurotransmitter amino acids, dopamine serotonin and their metabolities in the rat hippocampus

作　　者：贾健民[1] 贾健平 申玉[1] 贾琳[1] Pollock M 

机构地区：[1]邯郸市第一医院神经内科,056002 [2]Otago大学医学院神经内科

出　　处：《卒中与神经疾病》2000年第4期203-206,共4页Stroke and Nervous Diseases

摘　　要：目的：研究脑反复缺血后海马细胞外液氨基酸和单胺递质及其代谢产物的变化规律。方法：采用Ｐｕｌｓｉｎｅｌｌｉ和Ｂｒｉｅｒｌｅｙ ４血管闭塞的方法，使鼠脑反复缺血，海马微管透析及高压液相电化学检测，观察细胞外谷氨酸（Ｇｌｕ），天门冬氨酸（Ａｓｐ），谷氨酰胺，牛磺酸，丙氨酸，丝氨酸，多巴胺（ＤＡ），５－羟色胺（５－ＨＴ）及其代谢产物浓度的变化。结果：缺血期，Ｇｌｕ和Ａｓｐ骤然增高５０倍和３０倍。缺血期ＤＡ和５－ＨＴ含量分别增加３０倍和５０倍，随后逐渐下降，再灌注１００ｍｉｎ恢复到基线水平，与此同时，其酸性代谢产物３，４二羟苯乙酸（ＤＯＰＡＣ），高香草酸（ＨＶＡ），５－羟吲哚乙酸（５－ＨＩＡＡ）在缺血期明显下降。结论：缺血期海马细胞外液兴奋性氨基酸和单胺递质急剧大量释放并触发膜离子通道改变，Ｃａ２＋超载，自由基反应，共同介导神经元缺血死亡。Objective: To explore the changes of monoamine and excitatory amino acids on ischemic Hippocampus Methods: In repeated global ischemia induced by Pulsinelli and Brierley's 4-VO(4-vessel occlusion) rat model, hippocampal extracellular fluid levels of monoamine and excitatory amino acids were determined at different time by using intracerebral microdialysis, high performance liquid chromatography and electrochemical detection (HPLC-ECD ). Results: Compared with pre-ischemic level, there were remarkable increases in extracellular DA (30-fold ), 5-HT(50-fold ) and excitatory amino acide (50-fold for Glutamate, 30-fold for Aspartate and 1.8-fold for Serine) during period of ischemia,After 100 min of reperfusion they returned to normal . At the same time,the levels of DOPAC,HCA and 5-HIAA decreased. Conclusion: The neurotoxins overloaded calcium ant free radical reaction as a consequence of obvious increase of extracellular monoamines and excitatory amino acids in the hippocampus induced by ischemia are all contributory to the vulnerability of hippocampus to ischemia.

关 键 词：微管透析 海马 多巴胺 5-HT 脑缺血 

分 类 号：R743.3[医药卫生—神经病学与精神病学]