应用聚合酶链反应检测宫腔滋养细胞DNA的研究  

DETECTION OF DNA OF FETAL CELL FROM UTERINE CAVITY USING THE POLYMERASE CHINA REACTION

作　　者：王文勇[1] 张金环[2] 许倩[1] 李素婷[1] 王鲁华[1] 王志红[2] 

机构地区：[1]承德医学院生化教研室,河北承德067000 [2]承德医学院附属医院妇产科

出　　处：《承德医学院学报》2000年第4期106-108,共3页Journal of Chengde Medical University

摘　　要：目的 :依据孕妇宫腔内存在滋养细胞的理论 ,寻找一种非创伤性产前基因诊断的新方法。方法 :用聚合酶链反应 (PCR)对 1 5例孕 6～ 1 2周孕周初产妇宫腔胎儿脱落细胞的 DNA进行特异扩增 ,扩增的基因为 Y染色体短臂单拷贝基因片段 (DYS1 4) ,扩增片段的大小为2 39bp。结果 :8例妊娠男性胎儿中 7例出现特异扩增带 ,检出率为 87.5%。 7例妊娠女性胎儿未出现扩增带 ,无假阳性。本次研究的符合率为 93.3%。结论 :聚合酶链反应检测孕妇宫腔滋养细胞 DNA具有较高的灵敏度和特异性 ,孕早期宫腔冲洗作为非创伤性产前基因诊断的一种取材方法可应用于临床。Objective:To search for a new method of non-invasive prenatal diagnosis.Method:A single-copy human DYS14 gene of Y chromosome of fetal DNA sequence was amplified by Polymerase chain reaction(PCR).A 239 bp specific fragment was obtained.The fetal DNA samples of fifteen pregnant women were used for PCR.Resul[KG-8]ts:The fragment was identified in 7 of 8 male-bearing fetal DNA.The diagnostic rate was 87.5%.None of the other 7 female-bearing pregnant women had positive results.The final accuracy of 93.3% was attained in all cases.Conclusion:The finding of DNA of fetal cell from uterine cavity may have a new implication for non-invasive prenatal gene diagnosis,and the PCR has the advantages of sensitivity and spectificity which improve the clinical application.

关 键 词：产前诊断 聚合酶链反应 胎儿DNA 

分 类 号：R714.5[医药卫生—妇产科学]