骨形态发生蛋白-7对大鼠脑缺血再灌注损伤的保护作用  

Protective Effect of Bone Morphogenetic Protein-7 on Focal Cerebral Ischemia-Reperfusion Injury in Rats

作　　者：曹东明[1] 李晓慧[1] 李龙[2] 裴海涛[1] 

机构地区：[1]青岛大学医学院附属医院急诊神经科,山东青岛266003 [2]复旦大学附属中山医院泌尿外科,上海200032

出　　处：《中国临床医学》2013年第5期616-619,共4页Chinese Journal of Clinical Medicine

基　　金：山东省自然科学基金资助项目(编号:ZR2009CM121);青岛市科技局科研基金资助项目(编号:091-3-78-JCH)

摘　　要：目的:探讨骨形态发生蛋白-7(130ne morphogenetic protein-7,BMP-7)对大鼠脑缺血再灌注损伤的保护作用及其可能的作用机制。方法:采用线栓法建立大脑局灶性脑缺血再罐注损伤模型,按照随机数字表法将Wistar大鼠随机分为假手术组、模型组、BMP-7治疗组。在缺血2 h再灌注24 h后对各组进行神经功能评分。采用原位末端标记法(TUNEL法)检测皮质区神经细胞凋亡,采用实时定量-聚合酶链反应(RT-PCR)和蛋白质印迹法(Western b10tting)分别检测皮层区凋亡活化因子-1(apoptotic protease activating factor-1,Apaf-1)和Bcl-2/腺病毒E1 B 1 9kDa相互作用蛋白3(Bcl-2/adenovirus E1B 19 kDa interacting protein 3,BNIP3)的mRNA及蛋白的表达。结果:与模型组相比,BMP-7治疗组大鼠神经功能评分明显降低,TUNEL阳性细胞数减少,缺血侧皮层Apaf-1和BNIP3的mRNA和蛋白表达均降低。结论:BMP-7可能通过下调Apaf-1和BNIP3的表达抑制神经细胞凋亡,从而减轻脑缺血再灌注损伤。Objective：To observe the neuroprotective effects of bone morphogenetic protein-7（BMP-7） on focal cerebral ischemia-reperfusion injury in rats and the related mechanism.Methods：Focal cerebral ischemia-reperfusion model was established by inserting a monofilament thread into middle cerebral artery.The Wistar rats were randomly divided into sham-operated group,model group and BMP-7 injection group,according to random number table.Neurological deficiencies were evaluated at 24 h after ischemia-reperfusion.The neuronal apoptosis was assessed by TUNEL staining.The mRNA and protein expressions of apoptotic protease activating factor-1 （Apaf-1） and Bcl-2/adenovirus E1B 19 kDa interacting protein 3 （BNIP3） in cortex were determined by real-time polymerase chain reaction（RT-PCR） and Western blotting,respectively.Results：Compared with the model group,in BMP-7 injection group the neurological deficiency score was lower,the number of TUNEL positive cells decreased and the mRNA and protein levels of Apaf-1 and BNIP3 were lower.Conclusions：BMP-7 may play a protective effect on cerebral ischemia-reperfusion injury by inhibiting the expression of Apaf-1 and BNIP3 and suppressing the apoptosis of neurocytes.

关 键 词：脑缺血再灌注损伤 骨形态发生蛋白-7 凋亡活化因子-1 BCL-2 腺病毒E1B 19kDa相互作用蛋白3 

分 类 号：R743[医药卫生—神经病学与精神病学]