稀碱-浓盐法提取硫酸软骨素工艺条件的研究  

Study on extraction of chondroitin sulfate by dilute alkali-concentrated salt method

作　　者：付莹[1] 王鑫[1] 于童童[2] 徐丽萍[1] 

机构地区：[1]哈尔滨商业大学食品工程学院省高校食品科学与工程重点实验室,哈尔滨150076 [2]华中农业大学食品科技学院,武汉430070

出　　处：《哈尔滨商业大学学报（自然科学版）》2013年第5期553-557,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省研究生创新基金项目(YJSCX2012-141HLJ)

摘　　要：采用猪软骨为原料,以硫酸软骨素得率为指标,对稀碱-浓盐法提取硫酸软骨素的方法进行研究.选择碱液体积分数、提取温度、料液比和Na+浓度为影响因素,通过正交试验确定了稀碱-浓盐法提取硫酸软骨素的最佳条件为:碱液体积分数2.5%,料液比1∶6,Na+浓度2.5 mol/L,提取温度35℃,硫酸软骨素的得率为14.54%,通过紫外光谱检测确定提取物质为硫酸软骨素.Pig cartilage was used as raw material,the chondroitin sulfate extraction yield as the target to withdraw the method by dilute alkali-salt concentration.Influencing factors were selected which included alkali concentration,extraction temperature,solid-liquid ratio and Na＋ concentration,the best conditions were determined by orthogonal test extraction,the alkali concentration was 2.5 ％,the solid-liquid ratio was 1 ∶ 6 （g/mL）,the Na ＋ concentration was 2.5 mol/L,the extraction temperature was 40 ℃.Chondroitin sulfate was 14.54％.Determine the extraction of substances was chondroitin sulfate through the UV spectrum.

关 键 词：硫酸软骨素 稀碱-浓盐法 提取 

分 类 号：S2[农业科学—农业工程]