IFN-γ上调人胃癌细胞株PD-L1表达  

Interferon-γ up-regulates programmed death ligand 1 in human gastric cancer cells

作　　者：陈淑芬[1] 侯婧瑛[2] 吴淑云[1] 王凌云[1] 

机构地区：[1]中山大学孙逸仙纪念医院消化内科,广东广州510120 [2]中山大学孙逸仙纪念医院急诊科,广东广州510120

出　　处：《中国病理生理杂志》2013年第11期1952-1956,共5页Chinese Journal of Pathophysiology

基　　金：广东省科技社会发展项目(No.2012B031800362)

摘　　要：目的:研究不同人胃癌细胞株表面程序性死亡配体1(PD-L1)表达及干扰素γ(IFN-γ)对其表达的诱导情况。方法:分别培养不同人胃癌细胞株AGS、BGC823、MGC803和SGC7901,经不同浓度IFN-γ、作用不同时间后,利用流式细胞术及real-time PCR检测胃癌细胞株PD-L1在蛋白和mRNA水平的表达情况。结果:流式细胞术显示AGS、BGC823、MGC803和SGC7901细胞PD-L1蛋白表达率分别为(1.567±0.109)%、(2.640±0.577)%、(1.760±0.236)%和(16.030±1.289)%;不同胃癌细胞株对IFN-γ反应不同,经不同浓度IFN-γ刺激后均可不同程度上调PD-L1的表达(P<0.05);real-time PCR显示10μg/L IFN-γ刺激各胃癌细胞株12 h后PD-L1 mRNA水平上调(P<0.05)。结论:胃癌细胞株组成性表达PD-L1;IFN-γ可上调胃癌细胞株PD-L1的表达,呈现浓度、时间依赖性;其中以SGC7901细胞(来源于转移淋巴结)表达最高,推测胃癌细胞中PD-L1的表达与肿瘤分化程度无关,与组织来源和淋巴结转移有关;IFN-γ上调胃癌细胞PD-L1,表明IFN-γ并非都是抑制肿瘤增殖、生长,可能参与介导肿瘤免疫逃逸,故临床运用IFN-γ辅助治疗胃癌时应慎用。AIM： To study the expression of programmed death ligand 1 （PD-L1） in human gastric cancer cells with or without the stimulation of interferon-γ（IFN-γ）. MJETHODS： The protein levels of PD-L1 in 4 different human gastric cancer cell lines AGS, BGC823, MGC803 and SGC7901 with or without IFN-γtreatment were analyzed by flow cytometry. The mRNA expression of PD-L1 in those cell lines was also detected by real-time PCR. RESULTS ： Flow cytometry analysis showed that the rates of PD-L1 surface expression in the 4 human gastric cancer cell lines AGS, BGC823, MGC803 and SGC7901 were （1.567 ±0. 109）%, （2.640±0.577）%, （1. 760±0.236）% and （16. 030±1.289）%, respectively. After the 4 gastric cancer cell lines were treated with IFN-γ at different concentrations or for different time, the PD-L1 surface expression increased at different levels with significant differences between groups. Real-time PCR also indicated that IFN-γ up-regulated PD-L1 expression at mRNA level. CONCLUSION： PD-L1 surface expression is found in human gastric cancer cell lines AGS, BGC823, MGC803 and SGC7901. IFN-γ up-regulates the expression of PD-L1. SGC7901 cell line, which is from metastatic lymph nodes, expresses the highest protein level of PD-L1 among the 4 cell lines, indicating that PD-L1 expression may be related to lymph node metastasis, not to differentiation grade. IFN-γ, may mediate the tumor immune escape so that it should be carefully aoolied in the treatment of gastric cancer.

关 键 词：胃癌细胞 程序性死亡配体1 干扰素Γ 预后 

分 类 号：R363[医药卫生—病理学]