KLF16在肺腺癌中表达的临床意义及机制  

Clinical significance of KLF16 expression in patients with lung adenocarcinoma

作　　者：黄志宏[1] 骆文志[1] 蔡兴东[1] 

机构地区：[1]暨南大学附属第一医院呼吸内科,广东广州510632

出　　处：《中国病理生理杂志》2013年第11期1978-1983,共6页Chinese Journal of Pathophysiology

基　　金：暨南大学培育基金资助项目(No.21613312)

摘　　要：目的：探讨Krüppel样转录因子16（Krüppel-like transcription factor 16,KLF16）蛋白表达在肺腺癌患者中的临床意义及机制,为探索肺腺癌患者预后生物标志物提供理论依据.方法：收集我院50例肺腺癌患者的肿瘤组织标本,对其进行4～6年临床随访,免疫组织化学染色分析患者病理标本中KLF16蛋白的表达,探索KLF16蛋白表达与肺腺癌患者预后的关系及其意义.通过上调肺腺癌细胞中KLF16蛋白的表达,流式细胞术检测KLF16蛋白对肺腺癌细胞周期和凋亡的影响,证实KLF16蛋白在肺腺癌中表达的作用.结果：50例肺腺癌患者中,KLF16蛋白低表达或不表达者29例,占58%（29/50）,在肺腺癌细胞中,KLF16表达明显低于其在正常支气管上皮细胞中的表达.KLF16蛋白低表达的肺腺癌患者,其5年生存率明显低于高表达KLF16的患者（P＜0.01）,而且KLF16蛋白低表达是肺腺癌患者预后不良的重要预测指标.上调肺腺癌细胞中KLF16蛋白的表达可诱导肺腺癌细胞周期S期阻滞.结论：KLF16在肺腺癌中是一个重要的抑癌蛋白,可作为肺腺癌患者重要的预测预后的分子标志物.AIM： To investigate the clinical significance of KrUppel-like transcription factor 16 （KLFI6） pro- tein expression in the patients with lung adenocarcinoma for exploring the prognostic biomarkers for lung cancer. METH- ODS ： The tumor tissue samples from 50 cases of lung adenocarcinoma were collected from our hospital, and 4 ~ 6 years of follow-up of the patients was obtained. The protein expression of KLF16 in the specimens was assayed by immunohistochemical staining. The correlation between KLF16 protein expression and prognosis of the patients with lung adenocarcinoma was also analyzed. The KLF16 protein was up-regulated in lung adenocarcinoma cells A549 and SPC-AI by transfection with pcDNA3.1 （ ＋ ）-KLF16 plasmid, and the cell cycle and apoptosis of the A549 cells and SPC-A1 cells were determined by flow cytometry after transfection. RESULTS ： Twenty-nine cases of the lung adenocarcinoma patients showed low or no KLF16 protein expression in the total 50 cases （58%, 29/50）. Furthermore, the KLF16 expression in the lung adenocareinoma cells was significantly lower than that in normal bronchial epithelial cells. The 5-year survival rate of the lung adenocarcinoma patients with low expression of KLF16 protein was significantly lower than that of the patients with high ex- pression of KLF16 protein （P 〈 0.01 ）, and low expression of KLF16 protein was an important predictor of poor prognosis for the patients with lung adenocarcinoma. Up-regulation of KLF16 protein expression in lung adenocarcinoma cells induced the cell cycle arrest in S phase. CONCLUSION： KLF16 is an important tumor suppressor in lung adenocarcinoma, and it can be used as an important molecular marker for predicting the prognosis of the patients with lung adenocarcinoma.

关 键 词：肺肿瘤 Krtippel样转录因子16 肿瘤标志物 

分 类 号：R363[医药卫生—病理学]