脂肪组织基质血管组分移植对阿霉素诱导的心力衰竭大鼠心功能的影响  

Effects of transplantation of adipose stromal vascular fraction on cardiac function in adriamycin-induced heart failure rats

作　　者：庄伟[1] 谢良地[1] 许昌声[1] 王华军[1] 沈逸华[1] 陈明[1] 

机构地区：[1]福建医科大学附属第一医院,福建省高血压研究所,福建福州350005

出　　处：《中国病理生理杂志》2013年第11期2030-2033,共4页Chinese Journal of Pathophysiology

基　　金：福建医科大学苗圃科研基金资助项目(No.2010MP008);福建省卫生厅青年科研基金资助项目(No.2009-2-14)

摘　　要：目的:观察脂肪组织基质血管组分(SVF)静脉移植对阿霉素诱导的心力衰竭大鼠心功能的影响,并探讨其可能的机制。方法:胶原酶体外分离培养SD大鼠SVF,并用绿色荧光蛋白标记。雄性SD大鼠28只随机分为正常对照组(n=8)、阿霉素对照组(n=10)和SVF治疗组(n=10);15 mg/kg阿霉素腹腔注射,每周2次,连续4周,建立大鼠心力衰竭模型;SVF治疗组予以阴茎静脉移植SVF(1×107/L细胞0.5 mL),其余两组给予等量PBS注射。移植4周后,经导管多导生理仪测定心功能;冰冻切片荧光显微镜检测SVF在心肌组织中归巢情况;免疫组化法检测新生血管内皮标志物CD31的表达。结果:(1)与阿霉素对照组相比,SVF治疗组左室收缩压峰值[LVSP,(135.65±21.58)mmHg vs(105.58±22.62)mmHg,P<0.05]、左室压最大上升速率[+dp/dt max,(4 815.65±566.24)mmHg/s vs(3 535.50±465.28)mmHg/s,P<0.05]和左室压最大下降速率[-dp/dt max,(3 677.56±467.75)mmHg/s vs(2 738.65±512.51)mmHg/s,P<0.05]均显著增加,但仍低于正常对照组;(2)SVF治疗组CD31免疫组化显示细胞核染色及棕黄色颗粒沉着分布较阿霉素对照组均匀,每个视野的血管数显著增多。结论:SVF移植可明显改善阿霉素诱导的心力衰竭大鼠心功能,部分机制可能与其促进心肌组织血管再生有关。AIM： To investigate the effects of transplantation of adipose stromal vascular fraction （SVF） on the cardiac function in adriamycin-induced heart failure rats. METHODS ： SVF was isolated from adipose tissue of a SpragueDawley （SD） rat by collagenase digestion and marked with green fluorescent protein （GFP） in vitro. Twenty-eight SD rats were randomized into normal control group （ n = 8 ）, adriamycin control group （ n = 10） and SVF treatment group （ a = 10）. Adriamycin at dose of 15 mg/kg was intraperitoneally injected into the rats twice a week for 4 weeks to induce heart failure. SVF cells （0. 5 mL, 1 × 10^7/L） were injected via penis vein, and PBS vehicle was injected into the control animals in the same way. Four weeks later, the cardiac function was determined by multichannel physiologic recorder via cardiac catheterization. SVF was demonstrated in the myocardium by frozen section fluorescence microscopy. The CD31 expression was determined by an immunohistochemical test. RESULTS： Compared with adriamycin control group, SVF transplantation increased left ventricular peak systolic pressure [ LVSP, （ 135.65 ± 21.58 ） mmHg vs （ 105.58 ± 22. 62 ） mmHg, P 〈 0. 05 ], left ventricular pressure maximal rise rate [ ＋ dp/dtmax, （4 815.65 ± 566.24 ） mmHg/s vs （ 3 535.50 ± 465.28 ） mmHg/s, P 〈 0. 05 ], and left ventricular pressure maximal decline rate [ - dp/dt （3 677.56 ± 467.75） mmHg/s vs （2 738.65 ± 512. 51 ） mmHg/s, P 〈 0. 05]. The results of the CD31 immunohistoehemieal test showed that the nuclear staining and granule distribution were more uniform, and the number of blood vessels per visual field increased in SVF treatment group as compared with adriamycin control group （ P 〈 0. 05 ）. CONCLUSION ： SVFtransplantation improves the cardiac function in the rat model of heart failure, possibly and partly through the promotion of myocardial neovascularization.

关 键 词：基质血管组分 阿霉素 心力衰竭 血管内皮 大鼠 

分 类 号：R363[医药卫生—病理学]