吸烟对口腔黏膜细胞分泌人β-防御素的影响  

The influence of smoking on human beta-defensin production secrected by oral mucosal cells

作　　者：叶沛[1] 王文梅[1] 王翔[1] 黎景景 

机构地区：[1]南京大学口腔医学院.南京市口腔医院黏膜科,210008

出　　处：《实用口腔医学杂志》2013年第6期799-802,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金项目(编号:81070839);江苏省六大人才高峰(编号:2010-WS-004);南京市医学科技发展重点项目(编号:zkx10030)

摘　　要：目的:探讨吸烟对口腔黏膜细胞分泌β-防御素(hBD)的影响。方法:采用商业化可购的直接烟暴露装置,构建体外leuk1细胞吸烟模型,细胞吸烟时处于气液交换环境,将其分为吸烟组(吸入香烟烟雾5、10、20、40 min)和对照组(吸入含5%CO2空气10 min),采用酶联免疫吸附法检测hBD1、hBD2、hBD3的浓度。结果:hBD1在吸烟组表达明显低于对照组(P=0.004),hBD2在吸烟组表达高于对照组(P=0.012),而2组中hBD3分泌量差异无显著性。结论:吸烟可使口腔黏膜细胞分泌hBD发生改变,进而影响口腔黏膜免疫。Objective：To investigate the potential effects of cigarette smoke on the secretion of human beta-defensins（hBD） by oral mucosal cells.Methods：A commercially available smoking chamber was used to culture leuk1 cells at the air-liquid interface.Cells were divided into smoking group （exposed to cigarette smoke for 5,10,20 and 40 min respectively） and control group （exposed to 5％ CO2 for 10 min）.The level of hBD was detected by ELISA.Results：hBD1 in smoking group was significantly lower than in control group （P =0.004）,however hBD2 expression in smoking group increased（vs control,P =0.012）,and in the two groups the level of hBD3 had no statistical difference.Conclusion：Smoking may change hBD secretion by oral mucosal cells,and then influence the defense immunity of oral mucosa.

关 键 词：人β-防御素(hBD) 口腔白斑 吸烟 免疫防御 

分 类 号：R781.5[医药卫生—口腔医学]