3种种植用钛合金铸造前后的细胞毒性研究  

The cytotoxicity of three kinds of titanium alloys before and after casting

作　　者：赵惠[1] 李潇[2] 金柱坤[2] 杨凯[2] 

机构地区：[1]南方医科大学研究生学院,广州510515 [2]广州军区广州总医院口腔科,广州510010

出　　处：《口腔医学》2013年第11期729-732,共4页Stomatology

基　　金：全军医学科研"十二五"面上课题(CWS11J271);广东省产学研结合项目(2012B091100171)

摘　　要：目的通过细胞培养MTT(噻唑蓝)实验,评价铸造对钛合金生物相容性的影响。方法分别将3组钛合金铸造前后的材料加工制成直径13.5 mm,厚度1 mm的圆片,清洗消毒后制备浸提液培养细胞,培养1,3,5 d后分别观察细胞形态、测试各组细胞吸光度值。结果①铸造前与铸造后,3组材料细胞吸光度值比较,含铝、钒的C组比其他两组稍低(P>0.05);②3组材料铸造前后吸光度值比较,铸造后稍低于铸造前(P>0.05)。结论铸造对于钛合金生物相容性无影响,不影响临床应用。Objective To evaluate the biocompatibility of titanium alloy before and after casting by cell culture and MTT test. Meth-ods Three kinds of titanium alloys before and after casting were processed into wafers whose diameter was 13.5 mm and thickness was 1 ram. Extracts were prepared for cell culture after cleaning and disinfection. Observe cell morphology and test the cells＇ absorbance val-ue in each group after culturing for 1,3 and 5 days. Results ①Cell absorbance value of C group which contained aluminum and vana-dium was slightly lower than those of the other two groups no matter before or after casting（ P 〉 0.05 ）. ②Cell absorbance values of three groups after casting were slightly lower than those before casting （ P 〉 0.05）. Conclusions Casting has no effect on the biocom-patibility of titanium alloys. It doesn＇t affect clinical application,

关 键 词：铸造 种植 细胞毒性 钛 

分 类 号：R783.1[医药卫生—口腔医学]