p38 MAPK通路介导急性肺栓塞大鼠单核细胞趋化蛋白1表达的研究  

P38 MAPK Pathway Mediated Expression of Monocyte Chemotactic Protein-1 in Rats with Acute Pulmonary Embolism

作　　者：蔡学定[1] 陈淳[2] 丁城[1] 徐晓梅[1] 黄晓颖[1] 王良兴[1] 

机构地区：[1]浙江省温州医学院附属第一医院呼吸内科,325003 [2]浙江省人民医院呼吸内科,杭州310014

出　　处：《医学研究杂志》2013年第11期36-39,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(30871138)

摘　　要：目的探究急性肺栓塞后大鼠肺组织中MCP-1的表达及p38 MAPK信号通路对MCP-1的影响。方法通过自体血栓回输法复制大鼠急性肺栓塞模型,分成1、4、8h组。采用免疫组化法检测急性肺栓塞大鼠MCP-1、p38 MAPK磷酸化蛋白的表达,采用半定量RT-PCR检测急性肺栓塞大鼠肺组织MCP-1 mRNA的表达,采用Western blot检测急性肺栓塞大鼠肺组织p-p38 MAPK表达。结果免疫组化检测结果显示MCP-1、p-p38 MAPK在大鼠肺组织中有明显表达,RT-PCR、Western blot检测结果显示急性肺栓塞后大鼠肺组织中MCP-1 mRNA、p-p38 MAPK的表达水平随时间点有明显升高,且两者呈正相关。结论急性肺栓塞大鼠MCP-1表达水平明显升高,其机制可能是肺栓塞后p38 MAPK通路被激活实现的。Objective To explore the expression of the MCP - 1 and the effect of the p38 MAPK after the acute pulmonary embolism of rats. Methods Model of acute pulmonary embolism was replicated Thorugh inputing thrombus （jugular vein catheter inserted to the right pulmonary artery）. The group was divided into lh,4h,8h groups. Immunohistochemical method was used to detect MCP- 1 and the expression of p38 MAPK. Semiquantitative rt - PCR the mRNA of MCP - 1. Western blot was used to detect the expression of p38 MAPK. Results Immunohistochemical assay showed that MCP - 1, p38 MAPK had obvious expression in rat＇s lung tissue. Also,the Rt - PCR and Western Blot detection results showed that the MCP - 1 mRNA and p38 MAPK expression was significantly increased after a- cute pulmonary embolism. And both of them had positively relationships. Conclusion In acute pulmonary embolism in rat lung tissue, the expression of MCP - 1 was significantly increased. The mechanism maybe through activating p38 MAPK pathway.

关 键 词：肺栓塞 P38 单核细胞趋化蛋白1 

分 类 号：R743[医药卫生—神经病学与精神病学]