乳腺浸润性导管癌Mus81表达的检测及其意义  

Detection and Significance of Mus81 in Breast Invasive Ductal Carcinoma

作　　者：钱颖[1] 孙荷[2] 吕娟[2] 吴云路[1] 董学君[1,2] 

机构地区：[1]温州医科大学检验医学院,生命科学学院,325035 [2]浙江省绍兴市人民医院,浙江大学绍兴医院临床检验中心

出　　处：《医学研究杂志》2013年第11期71-74,共4页Journal of Medical Research

基　　金：浙江省医药卫生平台重点资助项目(2013ZDA024);浙江省重点科技创新团队(2012R10048-09);浙江省市共建重点学科(GJSX-010-003)

摘　　要：目的研究乳腺浸润性导管癌组织中Mus81的表达及意义。方法采用反转录-聚合酶链反应(RT-PCR)和Western blot检测39例乳腺浸润性导管癌病灶组织及其对应的癌旁组织、15例乳腺纤维腺瘤组织中Mus81 mRNA和蛋白的表达情况。结果乳腺浸润性导管癌病灶组织中Mus81阳性率为66.67%(26/39),低于癌旁组织(100%),差异有统计学意义(P<0.05)。乳腺纤维腺瘤组织中Mus81阳性率(100%)与癌旁组织无差别。乳腺浸润性导管癌病灶组织Mus81 mRNA和蛋白平均表达水平低于癌旁组织,差异有统计学意义(t=-6.546,P<0.01;t=-4.264,P<0.01);乳腺浸润性导管癌病灶组织Mus81mRNA和蛋白平均表达水平低于乳腺纤维腺瘤组织,差异有统计学意义(t=-6.117,P<0.01;t=-3.566,P<0.01);癌旁组织Mus81 mRNA和蛋白平均表达水平低于乳腺纤维腺瘤组织,差异无统计学意义(t=-0.519,P>0.05;t=-2.128,P>0.05)。Mus81表达水平与乳腺癌TNM分期有关,处于TNM分期0、Ⅰ期的乳腺浸润性导管癌组织Mus81 mRNA和蛋白平均表达水平高于Ⅱ、Ⅲ、Ⅳ期,差异有统计学意义(t=2.297,P<0.05;t=2.763,P<0.05);Mus81表达水平与患者年龄、肿瘤大小、腋窝淋巴结转移无关(P>0.05)。结论 Mus81在乳腺浸润性导管癌中表达下调且与肿瘤TNM分期相关,检测Mus81的表达情况有助对乳腺浸润性导管癌的病情评估。Objective To explore the expression and significance of Mus81 in breast invasive ductal carcinoma tissues. Methods The expression of Mus81 mRNA and protein was detected in 39 human breast invasive ductal carcinoma tissues and 39 paired paracancer- ous tissues as well as 15 breast fibroadenoma tissues by RT - PCR and Western blot. Results MusS1 was positive in 26 of 39 breast in- vasive ductal carcinoma tissues. The expression of Mus81 in breast invasive ductal carcinoma tissues tissues was significantly lower than in that of paracancerous tissues（ t = - 6. 546,P 〈0. 01 ;t = - 4. 264, P 〈0. 01 ）. Mus81 expression in breast invasive ductal carcinoma tis-

关 键 词：乳腺浸润性导管癌Mus81 RT—PCR WESTERN BLOT 

分 类 号：R737.9[医药卫生—肿瘤]